Sciatic Nerve Chronic Constriction Injury
Objective: Surgical induction of neuropathic pain through chronic constriction injury of the sciatic nerve by clamping with forceps
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Equipment1
Materials1
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Protocol Steps
Animal anesthesia
Rats were deeply anesthetized with sodium pentobarbital administered by intraperitoneal injection
Note: Dosage: 50 mg/kg body weight
View evidence from paper
“rats were deeply anesthetized with sodium pentobarbital (50 mg/kg body weight by intraperitoneal injection)”
Sciatic nerve exposure
The sciatic nerve in the right mid-thigh was surgically exposed
Note: Right side nerve was targeted
View evidence from paper
“the sciatic nerve in the right mid-thigh was exposed”
Chronic constriction injury via clamping
The exposed sciatic nerve was clamped with forceps three times for 10 seconds each at 10-second intervals
Note: Three consecutive clamping cycles performed
View evidence from paper
“the sciatic nerve in the right mid-thigh was exposed and clamped with a pair of forceps three times for 10 s each at 10-s intervals”
Post-operative housing
Animals were housed in controlled conditions on a 12:12 hour light/dark cycle with free access to food and water
Note: Standard laboratory housing conditions maintained
View evidence from paper
“Animals were housed in controlled conditions on a 12:12 h light/dark cycle, with free access to food and water”