Motor Coordination Testing
Objective: Assessment of motor coordination in brainstem- and cerebellum-damaged animals to evaluate behavioral recovery following exercise intervention
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Equipment1
Not specified • Not specified • Not mentioned • Not mentioned
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Protocol Steps
Exercise intervention via treadmill running
Laboratory rodents were submitted to treadmill running at a distance of 1 km per day
Note: Exercise was administered either before or after neurotoxin insult, or along progression of inherited neurodegeneration
View evidence from paper
“Laboratory rodents were submitted to treadmill running (1 km/d) either before or after neurotoxin insult”
Hippocampal insult induction
Domoic acid was administered to induce hippocampal injury in experimental animals
Note: This represents one of three injury models tested
View evidence from paper
“neurotoxin insult of the hippocampus (domoic acid)”
Brainstem insult induction
3-acetylpyridine was administered to induce brainstem injury in experimental animals
Note: This represents one of three injury models tested
View evidence from paper
“or the brainstem (3-acetylpyridine)”
Cerebellar neurodegeneration monitoring
Animals with inherited Purkinje cell degeneration were monitored along progression of neurodegeneration
Note: This represents one of three injury models tested
View evidence from paper
“or along progression of inherited neurodegeneration affecting the cerebellum (Purkinje cell degeneration)”
Motor coordination assessment
Motor coordination was assessed in brainstem- and cerebellum-damaged animals to evaluate behavioral recovery
Note: Animals showed normal or near to normal motor coordination following exercise intervention
View evidence from paper
“normal or near to normal motor coordination in brainstem- and cerebellum-damaged animals”
Spatial memory assessment
Spatial memory was assessed in hippocampal-injured mice to evaluate behavioral recovery
Note: Animals showed intact spatial memory following exercise intervention
View evidence from paper
“intact spatial memory in hippocampal-injured mice”
Anti-IGF-I antibody administration
Blocking anti-IGF-I antibody was administered subcutaneously to exercising animals to inhibit exercise-induced brain uptake of IGF-I
Note: This intervention abrogated the protective effects of exercise in all types of lesions
View evidence from paper
“subcutaneous administration of a blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I”
Neuronal impairment assessment
Neuronal impairment or loss was assessed in all injury types to determine neuroprotective effects of exercise
Note: Exercise blocked neuronal impairment or loss in all types of injuries
View evidence from paper
“exercise blocked neuronal impairment or loss in all types of injuries”