Motor Coordination Testing
Objective: Assessment of motor coordination in brainstem- and cerebellum-damaged animals to evaluate behavioral recovery following exercise intervention
This is a Motor Coordination Testing protocol using rodents as the model organism. The procedure involves 8 procedural steps, 1 equipment items, 3 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
rodents • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Exercise intervention via treadmill running • Hippocampal insult induction • Brainstem insult induction
Primary readouts
- Motor coordination recovery in brainstem-damaged animals
- Motor coordination recovery in cerebellum-damaged animals
- Spatial memory performance in hippocampal-injured mice
- Neuronal impairment or loss assessment
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Exercise intervention via treadmill running
Laboratory rodents were submitted to treadmill running at a distance of 1 km per day
Note: Exercise was administered either before or after neurotoxin insult, or along progression of inherited neurodegeneration
View evidence from paper
“Laboratory rodents were submitted to treadmill running (1 km/d) either before or after neurotoxin insult”
Hippocampal insult induction
Domoic acid was administered to induce hippocampal injury in experimental animals
Note: This represents one of three injury models tested
View evidence from paper
“neurotoxin insult of the hippocampus (domoic acid)”
Brainstem insult induction
3-acetylpyridine was administered to induce brainstem injury in experimental animals
Note: This represents one of three injury models tested
View evidence from paper
“or the brainstem (3-acetylpyridine)”
Cerebellar neurodegeneration monitoring
Animals with inherited Purkinje cell degeneration were monitored along progression of neurodegeneration
Note: This represents one of three injury models tested
View evidence from paper
“or along progression of inherited neurodegeneration affecting the cerebellum (Purkinje cell degeneration)”
Motor coordination assessment
Motor coordination was assessed in brainstem- and cerebellum-damaged animals to evaluate behavioral recovery
Note: Animals showed normal or near to normal motor coordination following exercise intervention
View evidence from paper
“normal or near to normal motor coordination in brainstem- and cerebellum-damaged animals”
Spatial memory assessment
Spatial memory was assessed in hippocampal-injured mice to evaluate behavioral recovery
Note: Animals showed intact spatial memory following exercise intervention
View evidence from paper
“intact spatial memory in hippocampal-injured mice”
Anti-IGF-I antibody administration
Blocking anti-IGF-I antibody was administered subcutaneously to exercising animals to inhibit exercise-induced brain uptake of IGF-I
Note: This intervention abrogated the protective effects of exercise in all types of lesions
View evidence from paper
“subcutaneous administration of a blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I”
Neuronal impairment assessment
Neuronal impairment or loss was assessed in all injury types to determine neuroprotective effects of exercise
Note: Exercise blocked neuronal impairment or loss in all types of injuries
View evidence from paper
“exercise blocked neuronal impairment or loss in all types of injuries”