Purkinje Cell Degeneration Model
Objective: Assessment of inherited neurodegeneration affecting the cerebellum and Purkinje cells along disease progression, and evaluation of exercise-induced neuroprotection mediated by circulating insulin-like growth factor I (IGF-I)
This is a Purkinje Cell Degeneration Model protocol using rodents as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
rodents • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Treadmill exercise training • Purkinje cell degeneration disease progression monitoring • Anti-IGF-I antibody administration
Primary readouts
- Recovery of behavioral performance
- Motor coordination in cerebellum-damaged animals
- Neuronal impairment or loss
- Brain damage extent
Key equipment and reagents
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Protocol Steps
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Treadmill exercise training
Laboratory rodents were submitted to treadmill running at a distance of 1 km per day
Note: Exercise was performed either before or after neurotoxin insult or along progression of inherited neurodegeneration
View evidence from paper
“Laboratory rodents were submitted to treadmill running (1 km/d) either before or after neurotoxin insult”
Purkinje cell degeneration disease progression monitoring
Animals with inherited neurodegeneration affecting the cerebellum and Purkinje cells were monitored along disease progression
Note: This represents the natural disease progression model being studied
View evidence from paper
“along progression of inherited neurodegeneration affecting the cerebellum (Purkinje cell degeneration)”
Anti-IGF-I antibody administration
Subcutaneous administration of blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I
Note: This was performed to determine whether neuroprotection by exercise is mediated by IGF-I
View evidence from paper
“subcutaneous administration of a blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I”
Behavioral performance assessment
Assessment of motor coordination and behavioral recovery in animals with cerebellar and Purkinje cell damage
Note: Animals were compared between exercise and sedentary groups
View evidence from paper
“animals show recovery of behavioral performance compared with sedentary ones, i.e., intact spatial memory in hippocampal-injured mice, and normal or near to normal motor coordination in brainstem- and cerebellum-damaged animals”
Neuronal assessment
Evaluation of neuronal impairment or loss in brain tissue
Note: Assessment performed across all types of lesions and disease conditions
View evidence from paper
“exercise blocked neuronal impairment or loss in all types of injuries”