Non-NMDA Antagonist Control Study
Objective: Control experiment to assess NMDA receptor specificity by testing whether non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione infused into the amygdala blocks extinction of conditioned fear
This is a Non-NMDA Antagonist Control Study protocol using Not explicitly stated in provided text as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 1 materials. Extracted from a 1992 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Prepare non-NMDA antagonist solution • Infuse non-NMDA antagonist into amygdala • Conduct fear-potentiated startle testing
Primary readouts
- Extinction of conditioned fear (blocked or not blocked)
- Startle response magnitude
- Effect of non-NMDA antagonist on fear extinction compared to NMDA antagonist
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Prepare non-NMDA antagonist solution
Prepare 6-cyano-7-nitroquinoxaline-2,3-dione at a selected dose for intra-amygdala infusion
Note: Specific dose not stated in provided text
View evidence from paper
“intra-amygdala infusion of a selected dose of the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione”
Infuse non-NMDA antagonist into amygdala
Administer the non-NMDA antagonist directly into the amygdala via intra-amygdala infusion
Note: This is a control experiment to test NMDA receptor specificity
View evidence from paper
“intra-amygdala infusion of a selected dose of the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione”
Conduct fear-potentiated startle testing
Employ the fear-potentiated startle paradigm to measure extinction of conditioned fear following non-NMDA antagonist infusion
Note: Measure whether non-NMDA antagonist blocks extinction, as comparison to NMDA antagonist effects
View evidence from paper
“the fear-potentiated startle paradigm was employed to begin to investigate neural mechanisms of extinction”