Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Novel Object Recognition Test
Objective: Long-term memory recognition assessment
This is a Novel Object Recognition Test protocol using Rat as the model organism. The procedure involves 7 procedural steps, 1 equipment items, 2 materials. Extracted from a 2025 paper published in Biol Trace Elem Res.
Model and subjects
Rat • Sprague-Dawley • male • 3- to 4-month-old • 24
Study window
~1 day study window | ~10 minutes hands-on
Core workflow
Training day setup • 24-hour delay period • Testing day setup
Primary readouts
- Number of approaches to both objects
- Investigating time for each object
- Discrimination ratio (DR)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Training day setup
Place two identical objects (A and B) in the arena and allow rat to explore for 5 minutes
View evidence from paper
“During the training day, the rat was permitted to explore two similar objects (A and B) placed in the arena for 5 min”
24-hour delay period
Wait 24 hours between training and testing phases
View evidence from paper
“On the testing day, after 24 h”
Testing day setup
Replace object B with novel object C, keeping object A in place
View evidence from paper
“the novel object C replaced object B, alongside object A”
Testing phase exploration
Allow rat to explore for 5 minutes during testing phase
View evidence from paper
“The rat was again given 5 min to explore”
Arena cleaning
Thoroughly clean the arena at the end of each trial
View evidence from paper
“A thorough arena cleaning was performed at the end of the trial”
Data recording
Record number of approaches to both objects and investigating time
View evidence from paper
“Approaches ( N ) to both objects and investigating time were recorded”
Calculate discrimination ratio
Calculate discrimination ratio using the formula DR = (N − F)/(N + F)
View evidence from paper
“the discrimination ratio ( DR ) was estimated using the formula DR = ( N − F )/( N + F )”