Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Novel Object Recognition Test
behavioralRatSprague-Dawley
Objective: Long-term memory recognition assessment
Protocol Steps
1
Training day setup
Place two identical objects (A and B) in the arena and allow rat to explore for 5 minutes
View evidence from paper
“During the training day, the rat was permitted to explore two similar objects (A and B) placed in the arena for 5 min”
2
24-hour delay period
Wait 24 hours between training and testing phases
View evidence from paper
“On the testing day, after 24 h”
3
Testing day setup
Replace object B with novel object C, keeping object A in place
View evidence from paper
“the novel object C replaced object B, alongside object A”
4
Testing phase exploration
Allow rat to explore for 5 minutes during testing phase
View evidence from paper
“The rat was again given 5 min to explore”
5
Arena cleaning
Thoroughly clean the arena at the end of each trial
View evidence from paper
“A thorough arena cleaning was performed at the end of the trial”
6
Data recording
Record number of approaches to both objects and investigating time
View evidence from paper
“Approaches ( N ) to both objects and investigating time were recorded”
7
Calculate discrimination ratio
Calculate discrimination ratio using the formula DR = (N − F)/(N + F)
View evidence from paper
“the discrimination ratio ( DR ) was estimated using the formula DR = ( N − F )/( N + F )”