Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures methods

[
  {
    "@context": "https://schema.org",
    "@type": "HowTo",
    "name": "Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures methods",
    "description": "Protocol for Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures methods",
    "step": [
      {
        "@type": "HowToStep",
        "position": 1,
        "name": "Motor deficits in Slc35a2 cKO mice worsen over time",
        "text": "Slc35a2 conditional knockout (cKO) mice suffer from motor deficits indicated by open field and locomotor habituation tests. (A) Postnatal day 30-60 randomly selected mice were subjected to a weekly 28-point NeuroScore test to measure overall motor function. Male cKO ( n = 5-6) mice exhibited severe ataxic gait and failed to climb a 45-degree slope when compared to their littermates ( n = 15-17; Friedman test, p =.037). There was no detectable difference between female cKO ( n = 5-6) and control mice ( n = 12-16). All other comparisons are not significant (ns). (B) Open field test supported motor deficits in both male and female cKO. In distance traveled (cm), there are significant differences in male cKO versus all other genotypes (two-way analysis of variance [ANOVA], F 1..."
      },
      {
        "@type": "HowToStep",
        "position": 2,
        "name": "Slc35a2 cKO mice have more severe induced seizures and exhibit spontaneous seizures",
        "text": "To evaluate seizure susceptibility in Slc35a2 cKO mice, both female and male mice were administered pentylenetetrazole (PTZ), a γ-aminobutyric acid antagonist, via intraperitoneal injection at a dose of 45 mg/kg body weight (Figure ). Female control ( n = 5) demonstrated tonic-clonic seizures (Racine = 4-6) but did not progress to tonic extension with respiratory arrest (Racine = 7). This was significantly different from littermate female cKO mice, whereas four of five cKO mice developed severe tonic-clonic seizures with respiratory arrest (Racine = 7; two-way ANOVA, F 1, 17 = 13, p =.0022; Figure ). Control male mice responded to the PTZ injection with neck jerking (Racine = 3) and short clonic seizures (Racine = 4) that did not evol..."
      },
      {
        "@type": "HowToStep",
        "position": 3,
        "name": "Slc35a2 cKO mice demonstrate hypomyelination throughout cortical layers of cortex and corpus callosum",
        "text": "Slc35a2 conditional knockout (cKO) mice express hypomyelination patterns throughout several timepoints in myelination. 2′,3′-Cyclic nucleotide-3′-phosphodiesterase (CNPase) immunohistochemistry was performed using 30-µm-thick, paraformaldehyde-fixed brain tissue. Two to three sections per animal were used as technical replicates, and one section was used as a secondary antibody control by omitting the primary antibody. (A-C) CNPase, an enzyme found in the myelin sheath, is abundant and widespread at postnatal day (P) 15, P21, and P60 in littermate control mice ( n = 3 per timepoint per sex). cKO mice ( n = 3 per timepoint per sex) had patches of hypomyelination throughout the motor cortex as well as corpus callosum (CC), anterior commissure (AC), lateral septal (LS), and caudate/putamen (CPu) re..."
      }
    ],
    "tool": [],
    "supply": [],
    "isBasedOn": {
      "@type": "ScholarlyArticle",
      "headline": "Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures",
      "datePublished": "2025",
      "author": [
        {
          "@type": "Person",
          "name": "Tiffany M. Bartel"
        },
        {
          "@type": "Person",
          "name": "Chaitali Ghosh"
        },
        {
          "@type": "Person",
          "name": "Kathryn Bisaha"
        },
        {
          "@type": "Person",
          "name": "Jean Khoury"
        },
        {
          "@type": "Person",
          "name": "Ashley Nemes-Baran"
        },
        {
          "@type": "Person",
          "name": "Ingmar Blumcke"
        },
        {
          "@type": "Person",
          "name": "Imad Najm"
        }
      ],
      "identifier": "10.1111/epi.18697"
    }
  },
  {
    "@context": "https://schema.org",
    "@type": "BreadcrumbList",
    "itemListElement": [
      {
        "@type": "ListItem",
        "position": 1,
        "name": "Experiments",
        "item": "https://replicatescience.com/experiments"
      },
      {
        "@type": "ListItem",
        "position": 2,
        "name": "Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures methods",
        "item": "https://replicatescience.com/experiments/olig2-8208-specific-loss-8208-of-8208-function-slc35a2-results-in-hypomyelination-and-spontaneous-seizures-methods-tiffany-m-bartel-pmc12861014/olig2-specific-loss-of-function-slc35a2-results-in-hypomyelination-and-spontaneo"
      }
    ]
  }
]