Experiments/Open-field Arena Assessment

Source Paper

Maternal care effects on the hippocampal transcriptome and anxiety-mediated behaviors in the offspring that are reversible in adulthood

Ian C. G. Weaver, Michael J. Meaney, Moshe Szyf

Proceedings of the National Academy of Sciences • 2006

DOI PMC

Open-field Arena Assessment

behavioralNot explicitly stated in provided textNot explicitly stated in provided text

Objective: Assessment of anxiety-mediated behavior and locomotor activity in an unfamiliar open-field arena following central infusion of saline vehicle, histone deacetylase inhibitor trichostatin A (TSA), or the essential amino acid l-methionine

This is a Open-field Arena Assessment protocol using Not explicitly stated in provided text as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 3 materials. Extracted from a 2006 paper published in Proceedings of the National Academy of Sciences.

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Replication Essentials

Model and subjects

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

Study window

Estimated timing pending

Core workflow

Central infusion of test compounds • Open-field arena assessment • Hippocampal tissue collection and transcriptome analysis

Primary readouts

Open-field behavior (anxiety-mediated behaviors and locomotor activity)
Hippocampal transcriptome gene expression patterns
Identification of genes regulated by maternal care
Reversibility of maternal care effects on behavior and gene expression

Key equipment and reagents

Open-field arena

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Protocol Steps

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Central infusion of test compounds

Adult offspring received central infusion of one of three treatments: saline vehicle (control), trichostatin A (TSA), or l-methionine

Not explicitly statedNot explicitly stated

Note: Route of administration (central infusion) specified but specific injection volume, concentration, and timing not provided

View evidence from paper

“Adult offspring were centrally infused with saline vehicle, the histone deacetylase inhibitor trichostatin A (TSA), or the essential amino acid l-methionine”