Open-field Arena Assessment
Objective: Assessment of anxiety-mediated behavior and locomotor activity in an unfamiliar open-field arena following central infusion of saline vehicle, histone deacetylase inhibitor trichostatin A (TSA), or the essential amino acid l-methionine
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not explicitly stated • Not explicitly stated • Not explicitly stated • Not explicitly stated
Materials3
Software1
Not explicitly stated • Not explicitly stated
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Protocol Steps
Central infusion of test compounds
Adult offspring received central infusion of one of three treatments: saline vehicle (control), trichostatin A (TSA), or l-methionine
Note: Route of administration (central infusion) specified but specific injection volume, concentration, and timing not provided
View evidence from paper
“Adult offspring were centrally infused with saline vehicle, the histone deacetylase inhibitor trichostatin A (TSA), or the essential amino acid l-methionine”
Open-field arena assessment
Animals were placed in an unfamiliar open-field arena to assess anxiety-mediated behavior and locomotor activity
Note: Specific behavioral measures recorded not detailed in provided text
View evidence from paper
“The animals were assessed in an unfamiliar open-field arena”
Hippocampal tissue collection and transcriptome analysis
Hippocampal tissue was collected from each animal and evaluated by microarray analysis to assess gene expression patterns
Note: Timing of tissue collection relative to behavioral testing not specified
View evidence from paper
“the hippocampal transcriptome of each animal was evaluated by microarray analysis”