Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Open Field Test
Objective: This test assesses animals' locomotor and exploratory behaviors
This is a Open Field Test protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Biol Trace Elem Res.
Model and subjects
Rat • Sprague-Dawley • male • 3- to 4-month-old • 24
Study window
~5 minutes hands-on
Core workflow
Place rat in arena • Allow exploration • Record behavioral measures
Primary readouts
- Lines crossed in peripheral squares
- Lines crossed in central squares
- Rearing frequency
- Grooming frequency (licking and scratching)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Place rat in arena
Place the rat at the peripheral square of the arena
View evidence from paper
“At the peripheral square, the rat was placed”
Allow exploration
Allow the rat to explore the arena for 5 minutes
View evidence from paper
“permitted to explore for 5 min”
Record behavioral measures
Observe and record lines crossed, rearing frequency, grooming frequency, excretion frequency, and freezing time
View evidence from paper
“Lines (N) were observed in both peripheral and central squares, rearing frequency, grooming (licking and scratching) frequency, excretion (defecation and urination) frequency, and time spent freezing”
Clean arena
Thoroughly clean the arena at the end of the trial
View evidence from paper
“A thorough arena cleaning was performed at the end of the trial”