Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Open Field Test
behavioralRatSprague-Dawley
Objective: This test assesses animals' locomotor and exploratory behaviors
Materials & Equipment Checklist
2 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
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Protocol Steps
1
Place rat in arena
Place the rat at the peripheral square of the arena
View evidence from paper
“At the peripheral square, the rat was placed”
2
Allow exploration
Allow the rat to explore the arena for 5 minutes
View evidence from paper
“permitted to explore for 5 min”
3
Record behavioral measures
Observe and record lines crossed, rearing frequency, grooming frequency, excretion frequency, and freezing time
View evidence from paper
“Lines (N) were observed in both peripheral and central squares, rearing frequency, grooming (licking and scratching) frequency, excretion (defecation and urination) frequency, and time spent freezing”
4
Clean arena
Thoroughly clean the arena at the end of the trial
View evidence from paper
“A thorough arena cleaning was performed at the end of the trial”