Source Paper
Chronic Organic Magnesium Supplementation Enhances Tissue-Specific Bioavailability and Functional Capacity in Rats: A Focus on Brain, Muscle, and Vascular Health
Koc B, Hosgorler F, Kandis S, Acikgoz B, Kizildag S et al.
Biol Trace Elem Res • 2025
Open-Field Test
behavioralRatSprague Dawley
Objective: Evaluate rodents' locomotor activity, exploratory behavior, and emotional behavior
Protocol Steps
1
Prepare testing arena
Set up black plexiglass box (100×100×50 cm) with floor divided into squares (75% edges, 25% center) and illuminate with 100 lx light
View evidence from paper
“The box floor was divided into equal squares (75% at the edges, 25% at the center) and illuminated with a light source (100 lx)”
2
Define thigmotaxis zone
Mark margin area as 5 cm from arena walls as thigmotaxis zone
View evidence from paper
“The Thigmotaxis zone (margin area) was defined as 5 cm from the arena walls”
3
Place rat in center
Place rats in the center of the field to start the test
View evidence from paper
“The rats were placed in the center of the field”
4
Record behavior
Record durations spent at edges and center area using video tracking system for 300 seconds
View evidence from paper
“the durations spent at the edges and in the center area of the field were recorded with the video tracking system for 300 s”