Source Paper
Chronic Organic Magnesium Supplementation Enhances Tissue-Specific Bioavailability and Functional Capacity in Rats: A Focus on Brain, Muscle, and Vascular Health
Koc B, Hosgorler F, Kandis S, Acikgoz B, Kizildag S et al.
Biol Trace Elem Res • 2025
Open-Field Test
Objective: Evaluate rodents' locomotor activity, exploratory behavior, and emotional behavior
This is a Open-Field Test protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2025 paper published in Biol Trace Elem Res.
Model and subjects
Rat • Sprague Dawley • Not specified • Adult • 38
Study window
Estimated timing pending
Core workflow
Prepare testing arena • Define thigmotaxis zone • Place rat in center
Primary readouts
- Total distance traveled
- Time spent in center area
- Time spent in margin areas
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Prepare testing arena
Set up black plexiglass box (100 × 100 × 50 cm) with floor divided into squares (75% edges, 25% center) and illuminate with 100 lx light
View evidence from paper
“The box floor was divided into equal squares (75% at the edges, 25% at the center) and illuminated with a light source (100 lx)”
Define thigmotaxis zone
Mark margin area as 5 cm from arena walls as thigmotaxis zone
View evidence from paper
“The Thigmotaxis zone (margin area) was defined as 5 cm from the arena walls”
Place rat in center
Place rats in the center of the field to start the test
View evidence from paper
“The rats were placed in the center of the field”
Record behavior
Record durations spent at edges and center area using video tracking system for 300 seconds
View evidence from paper
“the durations spent at the edges and in the center area of the field were recorded with the video tracking system for 300 s”