Source Paper
The intake of monosodium aspartate attenuates aggression induced by post-weaning social isolation in an ADHD rat model
Nishimura Y, Mustika D, Ueno S, Tominaga S, Shindo M et al.
J Physiol Sci • 2026
Open Field Test
Objective: To evaluate anxiety-like behavior in a novel environment
This is a Open Field Test protocol using Rat as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 2 materials. Extracted from a 2026 paper published in J Physiol Sci.
Model and subjects
Rat • SHR/Izm (spontaneously Hypertensive Rats) • Male • P60 (60 days old) • 12
Study window
~10 minutes hands-on
Core workflow
Test timing • Subject placement • Test duration
Primary readouts
- Total distance traveled
- Number of entries into the center area
- Duration spent in the center area
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Test timing
Conduct test during dark phase between 13:00 and 16:00
View evidence from paper
“during the dark phase, between 13:00 and 16:00”
Subject placement
Position rats at the center of the arena
View evidence from paper
“Rats were positioned at the center of a black circular arena”
Test duration
Observe and record behavior for 10 minutes
View evidence from paper
“over a 10-min period”
Video recording
Record behaviors using video camera under red dim light
View evidence from paper
“Behaviors recorded via a video camera”
Data analysis
Analyze recorded behaviors using Smart software automatic tracking system
View evidence from paper
“analyzed using the Smart software automatic tracking system”