Source Paper
The intake of monosodium aspartate attenuates aggression induced by post-weaning social isolation in an ADHD rat model
Nishimura Y, Mustika D, Ueno S, Tominaga S, Shindo M et al.
J Physiol Sci • 2026
Open Field Test
behavioralRatSHR/Izm (spontaneously Hypertensive Rats)
Objective: To evaluate anxiety-like behavior in a novel environment
Materials & Equipment Checklist
4 items
Protocol Steps
1
Test timing
Conduct test during dark phase between 13:00 and 16:00
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“during the dark phase, between 13:00 and 16:00”
2
Subject placement
Position rats at the center of the arena
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“Rats were positioned at the center of a black circular arena”
3
Test duration
Observe and record behavior for 10 minutes
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“over a 10-min period”
4
Video recording
Record behaviors using video camera under red dim light
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“Behaviors recorded via a video camera”
5
Data analysis
Analyze recorded behaviors using Smart software automatic tracking system
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“analyzed using the Smart software automatic tracking system”