Source Paper
From Struggling (With) Screening Tests to Mouse Models of Depression‐Relevant Neurobehavioral States
Pryce CR
Curr Protoc • 2026
Pavlovian Aversion Learning-Memory
Objective: To measure increased reactivity to acute threats through Pavlovian aversion learning-memory (PALM) as a neurobehavioral state relevant to depression and stress-related disorders
This is a Pavlovian Aversion Learning-Memory protocol using Mice as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 2 materials. Extracted from a 2026 paper published in Curr Protoc.
Model and subjects
Mice • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Setup arena configuration • Administer aversive stimulus • Enable escape response
Primary readouts
- Pavlovian aversion learning-memory (PALM) responses
- Freezing behavior duration
- Escape response latency
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Setup arena configuration
Place mice in arena with electrifiable floor and central divider allowing transfer between compartments
View evidence from paper
“allowing transfer between the left and right compartments”
Administer aversive stimulus
Deliver foot electroshock as the aversive stimulus for Pavlovian conditioning
View evidence from paper
“foot e‐shock was now the aversive stimulus”
Enable escape response
Allow compartmental transfer via barrier as the operant escape response
View evidence from paper
“with compartmental transfer via a barrier or doorway the operant escape response”
Measure aversion learning-memory
Assess Pavlovian aversion learning-memory through tone-foot shock pairing leading to freezing behavior
View evidence from paper
“tone‐foot shock pairing leading to freezing behavior as a paradigm with which to study Pavlovian aversive association learning‐memory”