Source Paper
Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice
Dong W, Liu D, Fu S, Zhang J, Chen X et al.
Adv Sci (Weinh) • 2025
Social Interaction Test
Objective: To assess social interaction behavior and social avoidance in mice as a measure of depressive-like behavior
This is a Social Interaction Test protocol using Mice as the model organism. The procedure involves 5 procedural steps. Extracted from a 2025 paper published in Adv Sci (Weinh).
Model and subjects
Mice • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Subject categorization after chronic social defeat stress • Social interaction ratio measurement • Time in interaction zone measurement
Primary readouts
- Social interaction ratio
- Time in interaction zone
- Time in corner zone
- Heat plot spatial analysis
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Subject categorization after chronic social defeat stress
Mice treated with CSDS were categorized into susceptible and resilient groups based on core characteristics of depression
View evidence from paper
“Based on the core characteristics of depression (anhedonia and social avoidance), mice treated with CSDS were categorized into two groups: susceptible and resilient mice.”
Social interaction ratio measurement
Measure the social interaction ratio between control and test groups
View evidence from paper
“Compared with the control group, susceptible mice showed a significantly reduced social interaction ratio”
Time in interaction zone measurement
Record time spent in the interaction zone during the test
View evidence from paper
“susceptible mice showed a significantly reduced social interaction ratio and time in the interaction zone”
Time in corner zone measurement
Record time spent in corner zones as indicator of avoidance behavior
View evidence from paper
“along with increased time in the corner zone”
Generate heat plot visualization
Create typical heat plot showing spatial distribution during social interaction test
View evidence from paper
“Typical heat plot (C), social interaction ratio (D), time in interaction zone (D), time in corner zone (E)”