Photocell Cage Motor Activity
Objective: Measurement of nonambulatory spontaneous motor activity and ambulatory locomotor activity in photocell cages following CRF infusion into the locus coeruleus
This is a Photocell Cage Motor Activity protocol using Not explicitly stated as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 1990 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated • Not explicitly stated • unknown • Not explicitly stated • Not explicitly stated
Study window
Estimated timing pending
Core workflow
CRF Infusion into Locus Coeruleus • Place Animal in Photocell Cage • Measure Motor Activity
Primary readouts
- Nonambulatory spontaneous motor activity (increased following CRF infusion)
- Ambulatory locomotor activity (not altered following CRF infusion)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
CRF Infusion into Locus Coeruleus
Infuse CRF directly into the locus coeruleus at specified dose
Note: Bilateral infusion was used in biochemical studies; dose of 100 ng used for photocell cage measurement
View evidence from paper
“infusion of CRF into the LC (100 ng) significantly increased nonambulatory spontaneous motor activity measured in photocell cages”
Place Animal in Photocell Cage
Place the infused animal into the photocell cage apparatus for activity measurement
Note: Timing of measurement relative to infusion not specified
View evidence from paper
“measured in photocell cages; ambulatory (i.e., locomotor) activity was not altered”
Measure Motor Activity
Record nonambulatory spontaneous motor activity and ambulatory locomotor activity using photocell detection
Note: Two distinct measures: nonambulatory spontaneous motor activity and ambulatory locomotor activity
View evidence from paper
“Results showed that infusion of CRF into the LC (100 ng) significantly increased nonambulatory spontaneous motor activity”