Plant growth improvement mediated by nitrate capture in co-composted biochar methods
Aim. Evidence-backed execution summary for Plant growth improvement mediated by nitrate capture in co-composted biochar methods from Plant growth improvement mediated by nitrate capture in co-composted biochar.
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human
Subject model for the experiment.
- Use
- confirm full cohort details in the source paper
Method comparison: Release of the biochar-captured nitrogen
reagent used in the protocol.
- Use
- The sequential-washing procedure included shaking (150 rpm) of 1.5 g biochar with 10-ml extracting solution on a horizontal rotating shaker, subsequent filtering to separate filtrate and particles, then repeating and shaking/filtering the same particles again (1) 1 h with distilled water, (2) 24...
Materials and Methods
The biochar was produced from woody chips (80% coniferous, 20% deciduous wood) by Carbon Terra (formerly German Charcoal GmbH) in vertical retorts. Pyrolysis treatment temperature was approx. 700 °C over a period of about 36 hours before passing a fire front at the end of the pyrolysis process. Ther...
- Use
- The biochar was produced from woody chips (80% coniferous, 20% deciduous wood) by Carbon Terra (formerly German Charcoal GmbH) in vertical retorts. Pyrolysis treatment temperature was approx. 700 °C over a period of about 36 hours before passing a fire front at the end of the pyrolysis process. Ther...
Plant growth study with composted (BC comp ) versus untreated (BC pure ) biochar
During cultivation the soil mixtures were daily adjusted to 65% of the maximum water holding capacity (WHC). For more detailed information see and and. The plants were kept in the vegetative-growth stage by repeatedly removing tiny emerging flowers to prevent seed formation, and thus termination of the vegetative g...
- Use
- During cultivation the soil mixtures were daily adjusted to 65% of the maximum water holding capacity (WHC). For more detailed information see and and. The plants were kept in the vegetative-growth stage by repeatedly removing tiny emerging flowers to prevent seed formation, and thus termination of the vegetative g...
Nutrient analyses of soil-biochar substrates, biochar particles and extracts
The produced compost and biochar-compost were analyzed by standardized methods in a commercial lab (Eurofins, Germany). Mineral nitrogen (i.e. NH 4 +, NO 3 - also referred to as N min ) and organic C and N concentrations (N org = N total - N min ) were determined colorimetrically in water or KCl extrac...
- Use
- The produced compost and biochar-compost were analyzed by standardized methods in a commercial lab (Eurofins, Germany). Mineral nitrogen (i.e. NH 4 +, NO 3 - also referred to as N min ) and organic C and N concentrations (N org = N total - N min ) were determined colorimetrically in water or KCl extrac...
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Materials and Methods
The biochar was produced from woody chips (80% coniferous, 20% deciduous wood) by Carbon Terra (formerly German Charcoal GmbH) in vertical retorts. Pyrolysis treatment temperature was approx. 700 °C over a period of about 36 hours before passing a fire front at the end of the pyrolysis process. There, highest treatment temperatures of approx. 850 °C were reached for a short final period (biochar properties: ). The initial biomass must have contained some adhering soil which was revealed by the examination of the biochar (BC pure ) with scanning electron microscope ( ), i.e. mineral phases on the carbon matrix must have been present before pyrolysis was carried out. Also, high concentrations of potentially redox active Fe and Mn phases on the sample were revealed ( ). (A more detailed characterization of the biochar before and after composting is the subject...
Compost and biochar-compost production
Production of compost with and without biochar (BC) was carried out in three replicated windrows (2.50 × 10 m) in April-May 2011 at the Ithaka Institute (former Delinat Institute) in Valais, Switzerland. The input materials consisted of animal manures, straw, rock powder, soil and mature compost ( ). The production followed the guidelines of aerobic quality composting with daily turnover of the compost windrows for two weeks, following turnover periods of three days for five more weeks. To three out of six windrows, 20% (vol/vol) woody biochar ( ) was added at the start. After mixing, both variants quickly reached 60-70 °C; temperatures were significantly higher in the biochar-compost during the thermophilic phase. After 6 weeks the composts approached maturity. Pot trials with 8 different plant species during late-summer and autumn 2011 confi...
Plant growth study with composted (BC comp ) versus untreated (BC pure ) biochar
During cultivation the soil mixtures were daily adjusted to 65% of the maximum water holding capacity (WHC). For more detailed information see and and. The plants were kept in the vegetative-growth stage by repeatedly removing tiny emerging flowers to prevent seed formation, and thus termination of the vegetative growth (otherwise the weight difference between undernourished and well-nourished plants would have been even larger); biomass was harvested 81 days after sowing. The plants were divided into shoots and leaves without inflorescences; root biomass was retrieved by washing on day 82. Dry leaves were milled to powder and the C and N concentrations were measured by CN analyzer (VarioMax CNS macro-element analyzer, Elementar Analytical Systems GmbH, Hanau, Germany).
Measurement outputs
What raw and processed outputs should exist?
The sequential washing procedure revealed that only 40% of the total amount of nitrate and ammonium of BC comp had been detected by EUF (,C and ): BC comp released in total 521...
- Raw artifact
- Per-sample or per-animal endpoint measurements collected during the experiment
- Processed artifact
- Structured table with cleaned measurements ready for comparison
- Reported as
- Summary statistics and between-group or across-timepoint comparisons
Analysis plan
How should the outputs become interpretable results?
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
inferred from protocolPreprocessing / cleaning
Three-way ANOVA was used for the plant harvest data; two-way ANOVA was deployed for other data sets (WHC, N leaching etc.) where only two factors applied; the Tukey post-hoc test was employed to evaluate differences among biochar treatments.
from paperScoring or quantification
Quantify the primary readouts for this experiment: The sequential washing procedure revealed that only 40% of the total amount of nitrate and ammonium of BC comp had been detected by EUF (,C and ): BC comp released in total 521....
from paperStatistical comparison
Three-way ANOVA was used for the plant harvest data; two-way ANOVA was deployed for other data sets (WHC, N leaching etc.) where only two factors applied; the Tukey post-hoc tes...; Compost addition and a higher fertilization significantly improved plant growth in all treatments (, ). However, adding co-composted biochar always caused the largest plant gro...; The sequential washing procedure revealed that only 40% of the total amount of nitrate and ammonium of BC comp had been detected by EUF (,C and ): BC comp released in total 521...
from paperReporting output
Report representative outputs alongside summary comparisons for The sequential washing procedure revealed that only 40% of the total amount of nitrate and ammonium of BC comp had been detected by EUF (,C and ): BC comp released in total 521....
inferred from protocolStructured statistical methods
Three-way ANOVA was used for the plant harvest data; two-way ANOVA was deployed for other data sets (WHC, N leaching etc.) where only two factors applied; the Tukey post-hoc tes...; Compost addition and a higher fertilization significantly improved plant growth in all treatments (, ). However, adding co-composted biochar always caused the largest plant gro...; The sequential washing procedure revealed that only 40% of the total amount of nitrate and ammonium of BC comp had been detected by EUF (,C and ): BC comp released in total 521...
source structuredSource and audit
What supports the facts on this page?
Evidence quotes (3)
The biochar was produced from woody chips (80% coniferous, 20% deciduous wood) by Carbon Terra (formerly German Charcoal GmbH) in vertical retorts. Pyrolysis treatment temperature was approx. 700 °C over a period of about 36 hours before passing a fire front at the end of the pyrolysis process. There, highest treatment temperatures of approx. 850 °C were reached for a short final period (biochar properties: ). The initial biomass must have contained some adhering soil which was revealed by the examination of the biochar (BC pure ) with scanning electron microscope ( ), i.e. mineral phases on the carbon matrix must have been present before pyrolysis was carried out. Also, high concentrations of potentially redox active Fe and Mn phases on the sample were revealed ( ). (A more detailed characterization of the biochar before and after composting is the subject of a further paper in preparation.) The biochar can be considered as a class 1 biochar as per the IBI standard (IBI = international biochar initiative), and premium quality as per the EBC standard (EBC = European biochar certificate); for biochar properties (BC pure ) see Ta...
Production of compost with and without biochar (BC) was carried out in three replicated windrows (2.50 × 10 m) in April-May 2011 at the Ithaka Institute (former Delinat Institute) in Valais, Switzerland. The input materials consisted of animal manures, straw, rock powder, soil and mature compost ( ). The production followed the guidelines of aerobic quality composting with daily turnover of the compost windrows for two weeks, following turnover periods of three days for five more weeks. To three out of six windrows, 20% (vol/vol) woody biochar ( ) was added at the start. After mixing, both variants quickly reached 60-70 °C; temperatures were significantly higher in the biochar-compost during the thermophilic phase. After 6 weeks the composts approached maturity. Pot trials with 8 different plant species during late-summer and autumn 2011 confirmed that both composts adhered to existing German/Swiss quality guidelines (plant biomass yields were on average 11% higher with biochar-compost than pure compost). The biochar-compost mostly had improved properties compared to the compost without biochar (either in tendency or significantly, e.g....
During cultivation the soil mixtures were daily adjusted to 65% of the maximum water holding capacity (WHC). For more detailed information see and and. The plants were kept in the vegetative-growth stage by repeatedly removing tiny emerging flowers to prevent seed formation, and thus termination of the vegetative growth (otherwise the weight difference between undernourished and well-nourished plants would have been even larger); biomass was harvested 81 days after sowing. The plants were divided into shoots and leaves without inflorescences; root biomass was retrieved by washing on day 82. Dry leaves were milled to powder and the C and N concentrations were measured by CN analyzer (VarioMax CNS macro-element analyzer, Elementar Analytical Systems GmbH, Hanau, Germany).
Machine-readable layer
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