Source Paper
Ashwagandha (Withania somnifera) in insulin resistance and metabolic syndrome: A literature review on mechanisms
Azimi E, Rameshrad M, Ghasemzadeh Rahbardar M, Hosseinzadeh H
Iran J Basic Med Sci • 2026
PTZ-induced defecation and urination test
behavioralNot specifiedNot specified
Objective: Test for anti-stress activity by measuring PTZ-induced defecation and urination responses
Materials & Equipment Checklist
3 items
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
1
PTZ administration
Administer PTZ to induce defecation and urination as a form of acute experimental stress
Not specified
View evidence from paper
“PTZ-induced defecation and urination, restraint stress-induced gastric ulcers”
2
Test compound administration
Administer ashwagandha active components (sitoindosides VIII and Withaferin-A) to assess anti-stress activity
Not specified
View evidence from paper
“active components of ashwagandha, such as sitoindosides VIII and Withaferin-A, have intense anti-stress action”
3
Measure stress response
Assess defecation and urination responses as indicators of acute stress
Not specified
View evidence from paper
“intense anti-stress action against acute forms of experimental stress, including PTZ-induced defecation and urination”