Source Paper
Ashwagandha (Withania somnifera) in insulin resistance and metabolic syndrome: A literature review on mechanisms
Azimi E, Rameshrad M, Ghasemzadeh Rahbardar M, Hosseinzadeh H
Iran J Basic Med Sci • 2026
PTZ-induced defecation and urination test
Objective: Test for anti-stress activity by measuring PTZ-induced defecation and urination responses
This is a PTZ-induced defecation and urination test protocol using Not specified as the model organism. The procedure involves 3 procedural steps, 3 materials. Extracted from a 2026 paper published in Iran J Basic Med Sci.
Model and subjects
Not specified • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
PTZ administration • Test compound administration • Measure stress response
Primary readouts
- Defecation response
- Urination response
- Anti-stress activity assessment
Key equipment and reagents
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Protocol Steps
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PTZ administration
Administer PTZ to induce defecation and urination as a form of acute experimental stress
View evidence from paper
“PTZ-induced defecation and urination, restraint stress-induced gastric ulcers”
Test compound administration
Administer ashwagandha active components (sitoindosides VIII and Withaferin-A) to assess anti-stress activity
View evidence from paper
“active components of ashwagandha, such as sitoindosides VIII and Withaferin-A, have intense anti-stress action”
Measure stress response
Assess defecation and urination responses as indicators of acute stress
View evidence from paper
“intense anti-stress action against acute forms of experimental stress, including PTZ-induced defecation and urination”