Rebound Feeding Test
Objective: Assessment of feeding response following fasting with GHSR antagonist administration to determine the role of ghrelin receptor signaling in rebound feeding
This is a Rebound Feeding Test protocol using mice and rats as the model organism. The procedure involves 3 procedural steps, 2 materials. Extracted from a 2006 paper published in Journal of Clinical Investigation.
Model and subjects
mice and rats • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Fasting period • GHSR antagonist administration • Measure rebound feeding response
Primary readouts
- Rebound feeding response magnitude following fasting
- Effect of GHSR antagonist on feeding behavior
- Comparison of feeding responses in ghrelin-deficient and GHSR-deficient mice versus wild-type controls
Key equipment and reagents
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Protocol Steps
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Fasting period
Subject animals to fasting to induce rebound feeding response
Note: Fasting is the stimulus for measuring rebound feeding response
View evidence from paper
“blunted rebound feeding following fasting”
GHSR antagonist administration
Deliver selective GHSR antagonist via intra-VTA injection to block ghrelin receptor signaling
Note: Administration is intra-VTA (into ventral tegmental area)
View evidence from paper
“intra-VTA delivery of a selective GHSR antagonist blocked the orexigenic effect of circulating ghrelin”
Measure rebound feeding response
Assess feeding behavior following fasting period with GHSR antagonist treatment
Note: Rebound feeding is blunted by GHSR antagonist administration
View evidence from paper
“blunted rebound feeding following fasting”