Rebound Feeding Test
Objective: Assessment of feeding response following fasting with GHSR antagonist administration to determine the role of ghrelin receptor signaling in rebound feeding
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
Fasting period
Subject animals to fasting to induce rebound feeding response
Note: Fasting is the stimulus for measuring rebound feeding response
View evidence from paper
“blunted rebound feeding following fasting”
GHSR antagonist administration
Deliver selective GHSR antagonist via intra-VTA injection to block ghrelin receptor signaling
Note: Administration is intra-VTA (into ventral tegmental area)
View evidence from paper
“intra-VTA delivery of a selective GHSR antagonist blocked the orexigenic effect of circulating ghrelin”
Measure rebound feeding response
Assess feeding behavior following fasting period with GHSR antagonist treatment
Note: Rebound feeding is blunted by GHSR antagonist administration
View evidence from paper
“blunted rebound feeding following fasting”