Source Paper
Chronic Organic Magnesium Supplementation Enhances Tissue-Specific Bioavailability and Functional Capacity in Rats: A Focus on Brain, Muscle, and Vascular Health
Koc B, Hosgorler F, Kandis S, Acikgoz B, Kizildag S et al.
Biol Trace Elem Res • 2025
Rotarod Performance Test
Objective: To assess motor performance and coordination by measuring the ability of animals to maintain balance on an accelerating rotating rod
This is a Rotarod Performance Test protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items. Extracted from a 2025 paper published in Biol Trace Elem Res.
Model and subjects
Rat • Sprague Dawley • Not specified • Adult • 38
Study window
~8 week study window
Core workflow
Training session • Set rotarod parameters • Conduct performance test
Primary readouts
- Last rpm at which animals could walk (motor performance)
- Latency to fall (time until loss of balance)
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Training session
Train animals to walk on the accelerating rotarod on the first day
View evidence from paper
“The animals were trained to walk on the accelerating rotarod on the first day”
Set rotarod parameters
Configure the rotating rod to linearly increase speed from 4 to 40 rpm over 180 seconds
View evidence from paper
“The speed of the rod was linearly increased from 4 to 40 rpm for 180 s”
Conduct performance test
Perform motor performance measurements once after 8 weeks of magnesium supplementation
View evidence from paper
“Motor performance measurements were made just once after 8 weeks of Mg supplementation”
Record performance
Time each walking performance until the animal loses balance and falls down using the counter timer
View evidence from paper
“The counter timed each walking performance on the rotarod until the animal lost balance and fell down”