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Second-Order Schedule of Reinforcement with AMPA/Kainate Receptor Antagonism — Louk J. M. J. Vanderschuren | ReplicateScience

Experiments/Second-Order Schedule of Reinforcement with AMPA/Kainate Receptor Antagonism

Source Paper

Involvement of the Dorsal Striatum in Cue-Controlled Cocaine Seeking

Louk J. M. J. Vanderschuren, Patricia Di Ciano, Barry J. Everitt

Journal of Neuroscience • 2005

Second-Order Schedule of Reinforcement with AMPA/Kainate Receptor Antagonism

behavioralNot explicitly stated in provided textNot explicitly stated in provided text

Objective: Evaluate the role of dopamine and glutamate transmission in the dorsal striatum in cue-controlled cocaine seeking under a second-order schedule of reinforcement, specifically assessing the effects of AMPA/kainate receptor antagonism

Materials & Equipment Checklist

7 items1 from ConductScience

Gather these items before starting the experiment. Check off items as you prepare.

Equipment2

Operant conditioning chamber or behavioral apparatus

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

Infusion apparatus for intradorsal striatal injection

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

Materials4

LY293558 (AMPA/kainate receptor antagonist)

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

AP-5 (NMDA receptor antagonist)

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

α-flupenthixol (dopamine receptor antagonist)

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

Cocaine

Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text

Software1

Not explicitly stated in provided text

Not explicitly stated in provided text • Not explicitly stated in provided text

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Protocol Steps

1

Establish baseline cocaine seeking under second-order schedule

Train subjects on second-order schedule of reinforcement where cocaine-associated cues serve as conditioned reinforcers

Not explicitly stated in provided textNot explicitly stated in provided text

Note: This establishes well-established drug seeking behavior that depends on reinforcing effects of cocaine-associated stimuli

View evidence from paper

“well established drug seeking that depends on the reinforcing effects of cocaine-associated stimuli”

2

Perform intradorsal striatal infusion of AMPA/kainate receptor antagonist

Infuse LY293558 into the dorsal striatum to block AMPA/kainate receptors

Not explicitly stated in provided textNot explicitly stated in provided text

Note: LY293558 is the specific AMPA/kainate receptor antagonist used

View evidence from paper

“intradorsal striatal infusion of the AMPA/kainate (KA) receptor antagonist LY293558”

3

Assess cue-controlled cocaine seeking following AMPA/kainate antagonism

Measure cocaine seeking behavior under second-order schedule after LY293558 infusion

Not explicitly stated in provided textNot explicitly stated in provided text

Note: AMPA/kainate antagonism decreased cue-controlled cocaine seeking

View evidence from paper

“intradorsal striatal infusion of the AMPA/kainate (KA) receptor antagonist LY293558, but not the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”

4

Perform intradorsal striatal infusion of dopamine receptor antagonist as comparison

Infuse α-flupenthixol into the dorsal striatum to block dopamine receptors

Not explicitly stated in provided textNot explicitly stated in provided text

Note: Used to compare dopamine's role with glutamate transmission

View evidence from paper

“Infusion of the DA receptor antagonist α-flupenthixol into the dorsal striatum decreased cocaine seeking under a second-order schedule of reinforcement”

5

Assess cue-controlled cocaine seeking following dopamine antagonism

Measure cocaine seeking behavior under second-order schedule after α-flupenthixol infusion

Not explicitly stated in provided textNot explicitly stated in provided text

Note: Dopamine antagonism also decreased cocaine seeking, similar to AMPA/kainate antagonism

View evidence from paper

“Infusion of the DA receptor antagonist α-flupenthixol into the dorsal striatum decreased cocaine seeking under a second-order schedule of reinforcement”

6

Perform intradorsal striatal infusion of NMDA receptor antagonist as control

Infuse AP-5 into the dorsal striatum to block NMDA receptors as a negative control

Not explicitly stated in provided textNot explicitly stated in provided text

Note: AP-5 did not decrease cue-controlled cocaine seeking, indicating specificity of AMPA/kainate effects

View evidence from paper

“the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”