Source Paper
Involvement of the Dorsal Striatum in Cue-Controlled Cocaine Seeking
Louk J. M. J. Vanderschuren, Patricia Di Ciano, Barry J. Everitt
Journal of Neuroscience • 2005
Second-Order Schedule of Reinforcement with AMPA/Kainate Receptor Antagonism
Objective: Evaluate the role of dopamine and glutamate transmission in the dorsal striatum in cue-controlled cocaine seeking under a second-order schedule of reinforcement, specifically assessing the effects of AMPA/kainate receptor antagonism
This is a Second-Order Schedule of Reinforcement with AMPA/Kainate Receptor Antagonism protocol using Not explicitly stated in provided text as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 4 materials. Extracted from a 2005 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Establish baseline cocaine seeking under second-order schedule • Perform intradorsal striatal infusion of AMPA/kainate receptor antagonist • Assess cue-controlled cocaine seeking following AMPA/kainate antagonism
Primary readouts
- Cocaine seeking behavior under second-order schedule of reinforcement
- Effects of AMPA/kainate receptor antagonism on cue-controlled cocaine seeking
- Effects of dopamine receptor antagonism on cocaine seeking
- Effects of NMDA receptor antagonism on cocaine seeking (control)
Key equipment and reagents
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Protocol Steps
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Establish baseline cocaine seeking under second-order schedule
Train subjects on second-order schedule of reinforcement where cocaine-associated cues serve as conditioned reinforcers
Note: This establishes well-established drug seeking behavior that depends on reinforcing effects of cocaine-associated stimuli
View evidence from paper
“well established drug seeking that depends on the reinforcing effects of cocaine-associated stimuli”
Perform intradorsal striatal infusion of AMPA/kainate receptor antagonist
Infuse LY293558 into the dorsal striatum to block AMPA/kainate receptors
Note: LY293558 is the specific AMPA/kainate receptor antagonist used
View evidence from paper
“intradorsal striatal infusion of the AMPA/kainate (KA) receptor antagonist LY293558”
Assess cue-controlled cocaine seeking following AMPA/kainate antagonism
Measure cocaine seeking behavior under second-order schedule after LY293558 infusion
Note: AMPA/kainate antagonism decreased cue-controlled cocaine seeking
View evidence from paper
“intradorsal striatal infusion of the AMPA/kainate (KA) receptor antagonist LY293558, but not the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”
Perform intradorsal striatal infusion of dopamine receptor antagonist as comparison
Infuse α-flupenthixol into the dorsal striatum to block dopamine receptors
Note: Used to compare dopamine's role with glutamate transmission
View evidence from paper
“Infusion of the DA receptor antagonist α-flupenthixol into the dorsal striatum decreased cocaine seeking under a second-order schedule of reinforcement”
Assess cue-controlled cocaine seeking following dopamine antagonism
Measure cocaine seeking behavior under second-order schedule after α-flupenthixol infusion
Note: Dopamine antagonism also decreased cocaine seeking, similar to AMPA/kainate antagonism
View evidence from paper
“Infusion of the DA receptor antagonist α-flupenthixol into the dorsal striatum decreased cocaine seeking under a second-order schedule of reinforcement”
Perform intradorsal striatal infusion of NMDA receptor antagonist as control
Infuse AP-5 into the dorsal striatum to block NMDA receptors as a negative control
Note: AP-5 did not decrease cue-controlled cocaine seeking, indicating specificity of AMPA/kainate effects
View evidence from paper
“the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”