Source Paper
Involvement of the Dorsal Striatum in Cue-Controlled Cocaine Seeking
Louk J. M. J. Vanderschuren, Patricia Di Ciano, Barry J. Everitt
Journal of Neuroscience • 2005
Second-Order Schedule of Reinforcement with NMDA Receptor Antagonism
Objective: Evaluate the role of NMDA receptor antagonism in the dorsal striatum on cue-controlled cocaine seeking under a second-order schedule of reinforcement
This is a Second-Order Schedule of Reinforcement with NMDA Receptor Antagonism protocol using Not explicitly stated in provided text as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 4 materials. Extracted from a 2005 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Establish baseline cocaine seeking under second-order schedule • Perform intradorsal striatal infusion of NMDA receptor antagonist AP-5 • Measure cocaine seeking behavior following AP-5 infusion
Primary readouts
- Cue-controlled cocaine seeking behavior under second-order schedule of reinforcement
- Effects of NMDA receptor antagonism on conditioned reinforcing properties of cocaine-associated stimuli
- Comparison of dopamine versus glutamate receptor involvement in drug seeking
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Establish baseline cocaine seeking under second-order schedule
Train subjects on second-order schedule of reinforcement with cocaine as primary reinforcer and environmental stimuli as conditioned reinforcers
Note: This establishes well-established drug seeking that depends on reinforcing effects of cocaine-associated stimuli
View evidence from paper
“Dorsal striatal DA transmission has been shown to be enhanced during cocaine seeking under a second-order schedule of reinforcement”
Perform intradorsal striatal infusion of NMDA receptor antagonist AP-5
Infuse AP-5 into the dorsal striatum to evaluate its role in cue-controlled cocaine seeking
Note: AP-5 is an NMDA receptor antagonist being tested for effects on cocaine seeking
View evidence from paper
“intradorsal striatal infusion of the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”
Measure cocaine seeking behavior following AP-5 infusion
Observe and quantify cue-controlled cocaine seeking responses after NMDA receptor antagonism
Note: Evaluate whether AP-5 affects the conditioned reinforcing properties of cocaine-associated stimuli
View evidence from paper
“intradorsal striatal infusion of the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”
Compare with DA receptor antagonist α-flupenthixol infusion
Perform parallel infusion of α-flupenthixol into dorsal striatum as comparison condition
Note: Allows comparison of dopamine versus glutamate receptor involvement
View evidence from paper
“Infusion of the DA receptor antagonist α-flupenthixol into the dorsal striatum decreased cocaine seeking under a second-order schedule of reinforcement”
Compare with AMPA/kainate receptor antagonist LY293558 infusion
Perform parallel infusion of LY293558 into dorsal striatum as additional comparison
Note: Evaluates differential roles of AMPA/kainate versus NMDA glutamate receptors
View evidence from paper
“intradorsal striatal infusion of the AMPA/kainate (KA) receptor antagonist LY293558, but not the NMDA receptor antagonist AP-5, also decreased cue-controlled cocaine seeking”