Source Paper
Mitochondrial protective effects of ellagic acid in a rat model of sporadic Alzheimer’s disease induced by STZ
Khodaei F, Namavar MR, Soutodeh N, Hadipour A, Asadi F et al.
Sci Rep • 2026
Shuttle Box Test
Objective: To assess memory retention and avoidance learning behavior in rats
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Protocol Steps
Training session setup
Introduce rat to the chamber with light and dark compartments separated by guillotine door
View evidence from paper
“the rat was introduced to all of the chamber after then learning”
Administer punishment
When rat enters dark space, deliver electrical foot shock of 1 mA for 1 second
View evidence from paper
“if the entrance to dark space received a punishing electrical foot-shock (1 mA for 1 s)”
Wait interval
Allow 24 hours between training and retention sessions
View evidence from paper
“In the training session, which was 24 h. before the retention session”
Retention test
Place rats in box under similar conditions but without electric shock
View evidence from paper
“rats were placed in the box with similar conditions but without electric shock”
Record latency
Measure and record the time rat stays in light space with maximum allowed time of 300 seconds
View evidence from paper
“the time of stay in light space was recorded. The maximum entry latency allowed in the retention sessions was 300 s”