Source Paper
Dopamine release from the locus coeruleus to the dorsal hippocampus promotes spatial learning and memory
Kimberly A. Kempadoo, Eugene V. Mosharov, Se Joon Choi, David Sulzer, Eric R. Kandel
Proceedings of the National Academy of Sciences • 2016
Spatial Object Recognition Task
Objective: Assessment of selective attention and spatial object recognition following optogenetically-induced dopamine release from locus coeruleus to dorsal hippocampus
This is a Spatial Object Recognition Task protocol using Not explicitly stated in provided text as the model organism. The procedure involves 2 procedural steps. Extracted from a 2016 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Optogenetic stimulation of LC axons • Behavioral assessment of selective attention and spatial object recognition
Primary readouts
- Dopamine release levels in dorsal hippocampus
- Selective attention performance
- Spatial object recognition performance
- D1/D5 receptor-mediated effects
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Optogenetic stimulation of LC axons
Photostimulation of locus coeruleus axons to induce dopamine release in dorsal hippocampus
Note: Photostimulation produces increase in dopamine release in dorsal hippocampus
View evidence from paper
“Photostimulation of LC axons produced an increase in dopamine release in the dorsal hippocampus”
Behavioral assessment of selective attention and spatial object recognition
Test selective attention and spatial object recognition following optogenetically-induced dopamine release
Note: Optogenetically induced release of dopamine from LC into dorsal hippocampus enhanced selective attention and spatial object recognition
View evidence from paper
“optogenetically induced release of dopamine from the LC into the dorsal hippocampus enhanced selective attention and spatial object recognition”