Source Paper
Mitochondrial protective effects of ellagic acid in a rat model of sporadic Alzheimer’s disease induced by STZ
Khodaei F, Namavar MR, Soutodeh N, Hadipour A, Asadi F et al.
Sci Rep • 2026
Step-down Test
Objective: Memory retention assessment in neurological disorders such as Alzheimer's disease using inhibitory avoidance
This is a Step-down Test protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2026 paper published in Sci Rep.
Model and subjects
Rat • Sprague Dawley • Male • Not specified • 10
Study window
Estimated timing pending
Core workflow
Setup apparatus • Conduct learning session • Conduct retention session
Primary readouts
- Reaction latency (seconds)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Setup apparatus
Place wooden platform in center of plastic box floor
View evidence from paper
“wooden platform (4 × 4 × 4 cm) placed at the center of the floor”
Conduct learning session
Perform learning trial with 1 second duration and 1 mA electric stimulation
View evidence from paper
“learning and retention sessions consisted of two trials, with each trial lasting 1 s and delivering an electric stimulation of 1 mA”
Conduct retention session
Perform retention trial with 1 second duration and 1 mA electric stimulation
View evidence from paper
“learning and retention sessions consisted of two trials, with each trial lasting 1 s and delivering an electric stimulation of 1 mA”
Measure latency
Record rat's reaction latency with criterion of more than 60 seconds and maximum cut-off of 300 seconds
View evidence from paper
“criterion for learning and retention was the latency of the rat's reaction, which had to be more than 60 s. An upper cut-off latency time of 300 s was considered”