Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions methods
Aim. Evidence-backed execution summary for Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions methods from Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions.
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mouse
Subject model for the experiment.
- Use
- confirm full cohort details in the source paper
Weight distribution and gait analysis
Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and the CatWalk system (Noldus). All measuring parameters are illustrated as ratio of the ipsilateral to the surgery (left) hindpaw (LH) over the...
- Use
- Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and the CatWalk system (Noldus). All measuring parameters are illustrated as ratio of the ipsilateral to the surgery (left) hindpaw (LH) over the...
Weight distribution and gait analysis
The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables complete automatic gait analysis of these static parameters as well as dynamic gait parameters such as stride length, stand duration, and swing...
- Use
- The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables complete automatic gait analysis of these static parameters as well as dynamic gait parameters such as stride length, stand duration, and swing...
"CatWalk"-based analysis
Duty cycle expresses the stand as a percentage of a step cycle (step cycle is the time between two consecutive initial contacts of the same paw. Step cycle = stand + swing). Duty cycle = stand/(stand + swing) × 100%.
- Use
- Duty cycle expresses the stand as a percentage of a step cycle (step cycle is the time between two consecutive initial contacts of the same paw. Step cycle = stand + swing). Duty cycle = stand/(stand + swing) × 100%.
"CatWalk"-based analysis
Mice were habituated to the CatWalk setup and allowed to cross the corridor for three sessions. On each testing day, animals were allowed to cross the corridor for three times.
- Use
- Mice were habituated to the CatWalk setup and allowed to cross the corridor for three sessions. On each testing day, animals were allowed to cross the corridor for three times.
Experimental design and groups
Behavioral testing was split in different cohorts because of the large number of behavioral tests and to avoid over-handling of the animals. We investigated four cohorts of animals per housing condition. One cohort was analyzed using stimulus-evoked behavioral tests (Coldplate and von Frey test) and DWB test. A seco...
- Use
- Behavioral testing was split in different cohorts because of the large number of behavioral tests and to avoid over-handling of the animals. We investigated four cohorts of animals per housing condition. One cohort was analyzed using stimulus-evoked behavioral tests (Coldplate and von Frey test) and DWB test. A seco...
Non-reflexive behavioral tests
We used the DWB system (Bioseb, Boulogne, France) for incapacitance testing in freely moving mice. The system consists of a Plexiglas enclosure (11 × 11 cm) with a floor composed of 1,936 pressure transducers. A digital camera was placed at one side of the enclosure. Mice were allowed to move...
- Use
- We used the DWB system (Bioseb, Boulogne, France) for incapacitance testing in freely moving mice. The system consists of a Plexiglas enclosure (11 × 11 cm) with a floor composed of 1,936 pressure transducers. A digital camera was placed at one side of the enclosure. Mice were allowed to move...
"CatWalk"-based analysis
The CatWalk XT version 10.6 gait analysis system (Noldus, Netherlands system) consists of an enclosed 1.3 m black corridor on a glass plate, which is illuminated inside with a green LED. This light is internally reflected, except at those areas where the animal makes contact with the glass plate. Wherever the...
- Use
- The CatWalk XT version 10.6 gait analysis system (Noldus, Netherlands system) consists of an enclosed 1.3 m black corridor on a glass plate, which is illuminated inside with a green LED. This light is internally reflected, except at those areas where the animal makes contact with the glass plate. Wherever the...
"CatWalk"-based analysis
We used the following parameters: Paw print area represents the surface of the complete print of a paw.
- Use
- We used the following parameters: Paw print area represents the surface of the complete print of a paw.
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Weight distribution and gait analysis
Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and the CatWalk system (Noldus). All measuring parameters are illustrated as ratio of the ipsilateral to the surgery (left) hindpaw (LH) over the healthy (right) hindpaw (RH).
Weight distribution and gait analysis
The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables complete automatic gait analysis of these static parameters as well as dynamic gait parameters such as stride length, stand duration, and swing phase among others.
Methods
All animals were housed with food and water ad libitum under a standard 12-h light/dark cycle (light on between 7 a.m.-7 p.m.) with regulated ambient temperature of ± 22℃ and at relative humidity of 40%-50%.
Experimental design and groups
All behavioral experiments started two weeks after arrival of the mice and behavioral tests were applied up to 84 days (12 weeks) following surgery. Behavioral tests of grouped and isolated animals were not performed in parallel.
"CatWalk"-based analysis
Swing phase is the duration of no contact of a paw with the glass plate in a step cycle.
"CatWalk"-based analysis
Stance is the duration of ground contact for a single paw.
Voluntary wheel running activity
Animals were placed individually in cages containing a running wheel and free access to food and water. We usually started this measurement in the morning around 9 a.m. for a total duration of 24 h. Unrestricted voluntary wheel running activity was digitally recorded using the AWM counter (Lafayette Instrument, Louisiana, USA), which uses an optical sensor to detect the total revolutions of the wheel and is connected to an USB Interface and PC running an AWM Software (Lafayette Instrument, Louisiana, USA).
Weight distribution and gait analysis
Using the DWB system, grouped SNI mice showed a significant decrease in the ratio of the hindpaw weight distribution between the ipsilateral and contralateral paw as compared to the basal paw distribution, and as compared to the sham mice at 1, 2, 3, and 10 weeks following surgery ( ). Similarly, we measured a significant drop in the ratio of the paw print area of SNI mice compared to basal and sham animals ( ). Owing to the high degree of comparability between results of the DWB and CatWalk system, we analyzed isolated SNI and sham animals solely with the CatWalk system. Figure 2. Changes in static weight bearing following SNI. Grouped animals were analyzed using the Dynamic weight bearing system (Bioseb). The ratio of the left over the right hindpaw is shown for (a) paw weight and (b) paw print area. N = 6 mice/group, p < 0.05 indicated by * as compared to...
Measurement outputs
What raw and processed outputs should exist?
Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and th...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables com...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
Duty cycle expresses the stand as a percentage of a step cycle (step cycle is the time between two consecutive initial contacts of the same paw. Step cycle = stand&#...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
Mice were habituated to the CatWalk setup and allowed to cross the corridor for three sessions. On each testing day, animals were allowed to cross the corridor for three times.
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
Analysis plan
How should the outputs become interpretable results?
Acquisition
Capture run-level gait data for each animal and preserve the timepoint or treatment labeling.
inferred from protocolPreprocessing / cleaning
For all measurements, data were calculated and presented as mean ± SEM.
from paperScoring or quantification
Quantify the primary readouts for this experiment: Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and th...; The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables com...; Duty cycle expresses the stand as a percentage of a step cycle (step cycle is the time between two consecutive initial contacts of the same paw. Step cycle = stand&#...; Mice were habituated to the CatWalk setup and allowed to cross the corridor for three sessions. On each testing day, animals were allowed to cross the corridor for three times..
from paperStatistical comparison
For all measurements, data were calculated and presented as mean ± SEM. Unless stated otherwise, two-way repeated measures ANOVA followed by post hoc Tukey̵...; Using the DWB system, grouped SNI mice showed a significant decrease in the ratio of the hindpaw weight distribution between the ipsilateral and contralateral paw as compared to...; These static weight parameters were compared using the CatWalk system where we found a significant reduction in paw intensity contact ( ) and paw print area ( ) in SNI mice at e...; Isolated SNI mice showed a significant decrease in paw intensity contact ( ) and paw print area ( ) during the whole observation period as compared to their basal paw-ratios and...
from paperReporting output
Report representative outputs alongside summary comparisons for Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and th..., The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables com..., Duty cycle expresses the stand as a percentage of a step cycle (step cycle is the time between two consecutive initial contacts of the same paw. Step cycle = stand&#..., Mice were habituated to the CatWalk setup and allowed to cross the corridor for three sessions. On each testing day, animals were allowed to cross the corridor for three times..
inferred from protocolStructured statistical methods
For all measurements, data were calculated and presented as mean ± SEM. Unless stated otherwise, two-way repeated measures ANOVA followed by post hoc Tukey̵...; Using the DWB system, grouped SNI mice showed a significant decrease in the ratio of the hindpaw weight distribution between the ipsilateral and contralateral paw as compared to...; These static weight parameters were compared using the CatWalk system where we found a significant reduction in paw intensity contact ( ) and paw print area ( ) in SNI mice at e...; Isolated SNI mice showed a significant decrease in paw intensity contact ( ) and paw print area ( ) during the whole observation period as compared to their basal paw-ratios and...
source structuredSource and audit
What supports the facts on this page?
Evidence quotes (8)
Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and the CatWalk system (Noldus). All measuring parameters are illustrated as ratio of the ipsilateral to the surgery (left) hindpaw (LH) over the healthy (right) hindpaw (RH).
The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables complete automatic gait analysis of these static parameters as well as dynamic gait parameters such as stride length, stand duration, and swing phase among others.
All animals were housed with food and water ad libitum under a standard 12-h light/dark cycle (light on between 7 a.m.-7 p.m.) with regulated ambient temperature of ± 22℃ and at relative humidity of 40%-50%.
All behavioral experiments started two weeks after arrival of the mice and behavioral tests were applied up to 84 days (12 weeks) following surgery. Behavioral tests of grouped and isolated animals were not performed in parallel.
Swing phase is the duration of no contact of a paw with the glass plate in a step cycle.
Stance is the duration of ground contact for a single paw.
Animals were placed individually in cages containing a running wheel and free access to food and water. We usually started this measurement in the morning around 9 a.m. for a total duration of 24 h. Unrestricted voluntary wheel running activity was digitally recorded using the AWM counter (Lafayette Instrument, Louisiana, USA), which uses an optical sensor to detect the total revolutions of the wheel and is connected to an USB Interface and PC running an AWM Software (Lafayette Instrument, Louisiana, USA).
Using the DWB system, grouped SNI mice showed a significant decrease in the ratio of the hindpaw weight distribution between the ipsilateral and contralateral paw as compared to the basal paw distribution, and as compared to the sham mice at 1, 2, 3, and 10 weeks following surgery ( ). Similarly, we measured a significant drop in the ratio of the paw print area of SNI mice compared to basal and sham animals ( ). Owing to the high degree of comparability between results of the DWB and CatWalk system, we analyzed isolated SNI and sham animals solely with the CatWalk system. Figure 2. Changes in static weight bearing following SNI. Grouped animals were analyzed using the Dynamic weight bearing system (Bioseb). The ratio of the left over the right hindpaw is shown for (a) paw weight and (b) paw print area. N = 6 mice/group, p < 0.05 indicated by * as compared to control group, † as compared to basal values within a group, two-way repeated measures ANOVA with post hoc Tukey test. All data points represent mean ± SEM.
Machine-readable layer
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