Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions methods

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    "name": "Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions methods",
    "description": "Protocol for Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions methods",
    "step": [
      {
        "@type": "HowToStep",
        "position": 1,
        "name": "Weight distribution and gait analysis",
        "text": "Additionally, we were interested to monitor changes in weight distribution and different gait parameters. We used two different measuring systems, the DWB system (Bioseb) and the CatWalk system (Noldus). All measuring parameters are illustrated as ratio of the ipsilateral to the surgery (left) hindpaw (LH) over the healthy (right) hindpaw (RH)."
      },
      {
        "@type": "HowToStep",
        "position": 2,
        "name": "Weight distribution and gait analysis",
        "text": "The DWB system (Bioseb) enables the investigation of static gait parameters, as paw weight distribution and paw print area, in freely moving mice. The CatWalk system enables complete automatic gait analysis of these static parameters as well as dynamic gait parameters such as stride length, stand duration, and swing phase among others."
      },
      {
        "@type": "HowToStep",
        "position": 3,
        "name": "Methods",
        "text": "All animals were housed with food and water ad libitum under a standard 12-h light/dark cycle (light on between 7 a.m.-7 p.m.) with regulated ambient temperature of ± 22℃ and at relative humidity of 40%-50%."
      },
      {
        "@type": "HowToStep",
        "position": 4,
        "name": "Experimental design and groups",
        "text": "All behavioral experiments started two weeks after arrival of the mice and behavioral tests were applied up to 84 days (12 weeks) following surgery. Behavioral tests of grouped and isolated animals were not performed in parallel."
      },
      {
        "@type": "HowToStep",
        "position": 5,
        "name": "\"CatWalk\"-based analysis",
        "text": "Swing phase is the duration of no contact of a paw with the glass plate in a step cycle."
      },
      {
        "@type": "HowToStep",
        "position": 6,
        "name": "\"CatWalk\"-based analysis",
        "text": "Stance is the duration of ground contact for a single paw."
      },
      {
        "@type": "HowToStep",
        "position": 7,
        "name": "Voluntary wheel running activity",
        "text": "Animals were placed individually in cages containing a running wheel and free access to food and water. We usually started this measurement in the morning around 9 a.m. for a total duration of 24 h. Unrestricted voluntary wheel running activity was digitally recorded using the AWM counter (Lafayette Instrument, Louisiana, USA), which uses an optical sensor to detect the total revolutions of the wheel and is connected to an USB Interface and PC running an AWM Software (Lafayette Instrument, Louisiana, USA)."
      },
      {
        "@type": "HowToStep",
        "position": 8,
        "name": "Weight distribution and gait analysis",
        "text": "Using the DWB system, grouped SNI mice showed a significant decrease in the ratio of the hindpaw weight distribution between the ipsilateral and contralateral paw as compared to the basal paw distribution, and as compared to the sham mice at 1, 2, 3, and 10 weeks following surgery ( ). Similarly, we measured a significant drop in the ratio of the paw print area of SNI mice compared to basal and sham animals ( ). Owing to the high degree of comparability between results of the DWB and CatWalk system, we analyzed isolated SNI and sham animals solely with the CatWalk system. Figure 2. Changes in static weight bearing following SNI. Grouped animals were analyzed using the Dynamic weight bearing system (Bioseb). The ratio of the left over the right hindpaw is shown for (a) paw weight and (b) paw print area. N = 6 mice/group, p < 0.05 indicated by * as compared to..."
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    "isBasedOn": {
      "@type": "ScholarlyArticle",
      "headline": "Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions",
      "datePublished": "2016",
      "author": [
        {
          "@type": "Person",
          "name": "Claudia Pitzer"
        },
        {
          "@type": "Person",
          "name": "Rohini Kuner"
        },
        {
          "@type": "Person",
          "name": "Anke Tappe-Theodor"
        }
      ],
      "identifier": "10.1177/1744806916656635"
    }
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        "name": "Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions methods",
        "item": "https://replicatescience.com/experiments/voluntary-and-evoked-behavioral-correlates-in-neuropathic-pain-states-under-different-social-housing-conditions-methods-claudia-pitzer-pmc4956152/voluntary-and-evoked-behavioral-correlates-in-neuropathic-pain-states-under-different-social-housing-mlph9b44"
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