Y Maze
Objective: Assessment of hippocampal-dependent spatial learning task to evaluate the impact of hippocampal neurogenesis ablation on spatial cognition
This is a Y Maze protocol using Not explicitly stated in provided text as the model organism. The procedure involves 3 procedural steps, 2 equipment items. Extracted from a 2006 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Hippocampal Neurogenesis Ablation - Method 1 • Hippocampal Neurogenesis Ablation - Method 2 • Y Maze Spatial Learning Task
Primary readouts
- Performance on Y maze spatial learning task
- Contextual fear conditioning response
- Cued conditioning response
- Synaptic plasticity in dentate gyrus
Key equipment and reagents
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Protocol Steps
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Hippocampal Neurogenesis Ablation - Method 1
Focal X irradiation of the hippocampus performed on subjects
Note: One of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells impaired contextual fear conditioning”
Hippocampal Neurogenesis Ablation - Method 2
Genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells performed on subjects
Note: One of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells impaired contextual fear conditioning”
Y Maze Spatial Learning Task
Subjects are tested on the Y maze to assess hippocampal-dependent spatial learning
Note: This task was unaffected by hippocampal neurogenesis ablation
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected.”