A tale of two transmitters: serotonin and histamine as in vivo biomarkers of chronic stress in mice

Hersey M, Reneaux M, Berger SN, et al. Journal of neuroinflammation 2022;19(1):167.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Forced swim test

Setting	Value	Paper’s words
Water temperature, water depth, cylinder size	Cited to other work	“A forced swim test (FST) was conducted as described in Yankelevitch-Yahav et al. [32]”
Session duration	6 min	“behavior was observed for 6 min total”
Other settings in the paper
Pretest	first 2 min	“the first 2 min were classified as pretest”
Test	remaining 4 min	“remaining 4 min were the test”
Apparatus
Containers		“mice were placed in containers filled with water”
Noldus EthoVision		“Rodent behavior was analyzed using Noldus EthoVision (Leesburg, VA, USA)”
Methods cited to other work
Forced swim test method, including water temperature, water depth and container size	Yankelevitch-Yahav et al. [32]
Behavioral testing for anxiety and depressive-like phenotypes	[30]	“animals underwent previously established behavioral testing for anxiety and depressive-like phenotypes [30]”

Setting	Value	Paper’s words
Sucrose solution	1%	“water with a 1% sucrose solution”
Water was removed	midnight	“Water was removed at midnight”
Water and water/sucrose was placed	7:00	“water and water/sucrose was placed at 7:00”
Weighed	before administration	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
Weighed	1 h after placement (8:00)	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
Weighed	3 h after placement (11:00)	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
Weighed	12 h after placement (19:00)	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
The test was completed	twice	“The test was completed twice, first as a pretest and second as the test”
Apparatus
Bottles		“mice were exposed to bottles with water and water with a 1% sucrose solution”
Methods cited to other work
Behavioral testing for anxiety and depressive-like phenotypes	[30]	“animals underwent previously established behavioral testing for anxiety and depressive-like phenotypes [30]”

Hersey M, Reneaux M, Berger SN, et al. Journal of neuroinflammation 2022;19(1):167.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Setting	Value	Paper’s words
Water temperature, water depth, cylinder size	Cited to other work	“A forced swim test (FST) was conducted as described in Yankelevitch-Yahav et al. [32]”
Session duration	6 min	“behavior was observed for 6 min total”
Other settings in the paper
Pretest	first 2 min	“the first 2 min were classified as pretest”
Test	remaining 4 min	“remaining 4 min were the test”
Apparatus
Containers		“mice were placed in containers filled with water”
Noldus EthoVision		“Rodent behavior was analyzed using Noldus EthoVision (Leesburg, VA, USA)”
Methods cited to other work
Forced swim test method, including water temperature, water depth and container size	Yankelevitch-Yahav et al. [32]
Behavioral testing for anxiety and depressive-like phenotypes	[30]	“animals underwent previously established behavioral testing for anxiety and depressive-like phenotypes [30]”

Setting	Value	Paper’s words
Sucrose solution	1%	“water with a 1% sucrose solution”
Water was removed	midnight	“Water was removed at midnight”
Water and water/sucrose was placed	7:00	“water and water/sucrose was placed at 7:00”
Weighed	before administration	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
Weighed	1 h after placement (8:00)	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
Weighed	3 h after placement (11:00)	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
Weighed	12 h after placement (19:00)	“water and water/sucrose solutions were weighed before administration, 1 h after placement (8:00), 3 h after placement (11:00), and 12 h after placement (19:00)”
The test was completed	twice	“The test was completed twice, first as a pretest and second as the test”
Apparatus
Bottles		“mice were exposed to bottles with water and water with a 1% sucrose solution”
Methods cited to other work
Behavioral testing for anxiety and depressive-like phenotypes	[30]	“animals underwent previously established behavioral testing for anxiety and depressive-like phenotypes [30]”