Source Paper
Aberrant Hippocampal Activity Underlies the Dopamine Dysregulation in an Animal Model of Schizophrenia
Daniel J. Lodge, Anthony A. Grace
Journal of Neuroscience • 2007
Amphetamine-Induced Locomotor Activity
Objective: Measurement of locomotor responses to amphetamine administration in MAM-treated and control rats to assess dopamine system responsivity and evaluate the relationship between hippocampal dysfunction and dopamine system hyper-responsivity
This is a Amphetamine-Induced Locomotor Activity protocol using rat as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Adult • Not specified
Study window
Estimated timing pending
Core workflow
Prenatal MAM treatment • Amphetamine administration and locomotor measurement • Ventral hippocampus inactivation (in MAM-treated rats)
Primary readouts
- Amphetamine-induced locomotor activity in MAM-treated versus control rats
- Effect of ventral hippocampus inactivation on amphetamine-induced locomotor behavior
- Spontaneously firing ventral tegmental dopamine neuron population activity
Key equipment and reagents
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Prenatal MAM treatment
Administer methylazoxymethanol acetate prenatally to disrupt normal development and create the animal model of schizophrenia
Note: Creates MAM-treated group with altered dopamine system responsivity
View evidence from paper
“Adult rats in which development was disrupted by prenatal methylazoxymethanol acetate (MAM) administration”
Amphetamine administration and locomotor measurement
Administer amphetamine to adult MAM-treated and control rats and measure resulting locomotor activity responses
Note: MAM-treated rats display significantly greater amphetamine-induced locomotor behavior compared to controls
View evidence from paper
“normalized the augmented amphetamine-induced locomotor behavior”
Ventral hippocampus inactivation (in MAM-treated rats)
Inactivate the ventral hippocampus in MAM-treated rats to assess its role in dopamine system dysregulation
Note: Inactivation completely reversed elevated dopamine neuron population activity and normalized amphetamine-induced locomotor behavior
View evidence from paper
“vHipp inactivation completely reversed the elevated DA neuron population activity and also normalized the augmented amphetamine-induced locomotor behavior”