Source Paper
Sleep Deprivation Causes Behavioral, Synaptic, and Membrane Excitability Alterations in Hippocampal Neurons
Carmel M. McDermott, Gerald J. LaHoste, Chu Chen, Alberto Musto, Nicolas G. Bazan et al.
Journal of Neuroscience • 2003
Amygdala-dependent Learning Task
Objective: To determine the specificity of sleep deprivation effects to hippocampal function by testing amygdala-dependent learning as a control task, comparing performance between sleep-deprived and control rats
This is a Amygdala-dependent Learning Task protocol using rat as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2003 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~144 hours hands-on
Core workflow
Sleep Deprivation Protocol • Amygdala-dependent Learning Task • Performance Assessment
Primary readouts
- Performance on amygdala-dependent learning task
- Comparison of amygdala-dependent learning between sleep-deprived and control rats
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Sleep Deprivation Protocol
Expose rats to 72 hours of primarily rapid eye movement sleep deprivation using multiple sleep deprivation methods
Note: Multiple sleep deprivation methods were used to differentiate effects of sleep deprivation from nonspecific stress induced by sleep deprivation methods
View evidence from paper
“exposure of rats to 72 hr of primarily rapid eye movement SD”
Amygdala-dependent Learning Task
Administer amygdala-dependent learning task to sleep-deprived rats to assess whether sleep deprivation affects amygdala-dependent learning
Note: This task serves as a control to determine specificity of sleep deprivation effects to hippocampal function
View evidence from paper
“had no effect on an amygdala-dependent learning task”
Performance Assessment
Evaluate and compare performance on the amygdala-dependent learning task between sleep-deprived and control rats
Note: Results should show no impairment in amygdala-dependent learning if sleep deprivation effects are specific to hippocampal function
View evidence from paper
“had no effect on an amygdala-dependent learning task”