Source Paper
Sleep Deprivation Causes Behavioral, Synaptic, and Membrane Excitability Alterations in Hippocampal Neurons
Carmel M. McDermott, Gerald J. LaHoste, Chu Chen, Alberto Musto, Nicolas G. Bazan et al.
Journal of Neuroscience • 2003
Amygdala-dependent Learning Task
Objective: To determine the specificity of sleep deprivation effects to hippocampal function by testing amygdala-dependent learning as a control task, comparing performance between sleep-deprived and control rats
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Sleep Deprivation Protocol
Expose rats to 72 hours of primarily rapid eye movement sleep deprivation using multiple sleep deprivation methods
Note: Multiple sleep deprivation methods were used to differentiate effects of sleep deprivation from nonspecific stress induced by sleep deprivation methods
View evidence from paper
“exposure of rats to 72 hr of primarily rapid eye movement SD”
Amygdala-dependent Learning Task
Administer amygdala-dependent learning task to sleep-deprived rats to assess whether sleep deprivation affects amygdala-dependent learning
Note: This task serves as a control to determine specificity of sleep deprivation effects to hippocampal function
View evidence from paper
“had no effect on an amygdala-dependent learning task”
Performance Assessment
Evaluate and compare performance on the amygdala-dependent learning task between sleep-deprived and control rats
Note: Results should show no impairment in amygdala-dependent learning if sleep deprivation effects are specific to hippocampal function
View evidence from paper
“had no effect on an amygdala-dependent learning task”