Source Paper
Angelo Scuteri, Serena Sanna, Wei-Min Chen, Manuela Uda, Giuseppe Albai et al.
PLoS Genetics • 2007
The obesity epidemic is responsible for a substantial economic burden in developed countries and is a major risk factor for type 2 diabetes and cardiovascular disease. The disease is the result not only of several environmental risk factors, but also of genetic predisposition. To take advantage of recent advances in gene-mapping technology, we executed a genome-wide association scan to identify genetic variants associated with obesity-related quantitative traits in the genetically isolated population of Sardinia. Initial analysis suggested that several SNPs in the FTO and PFKP genes were associated with increased BMI, hip circumference, and weight. Within the FTO gene, rs9930506 showed the strongest association with BMI (p = 8.6 x10(-7)), hip circumference (p = 3.4 x 10(-8)), and weight (p = 9.1 x 10(-7)). In Sardinia, homozygotes for the rare "G" allele of this SNP (minor allele frequency = 0.46) were 1.3 BMI units heavier than homozygotes for the common "A" allele. Within the PFKP gene, rs6602024 showed very strong association with BMI (p = 4.9 x 10(-6)). Homozygotes for the rare "A" allele of this SNP (minor allele frequency = 0.12) were 1.8 BMI units heavier than homozygotes for the common "G" allele. To replicate our findings, we genotyped these two SNPs in the GenNet study. In European Americans (N = 1,496) and in Hispanic Americans (N = 839), we replicated significant association between rs9930506 in the FTO gene and BMI (p-value for meta-analysis of European American and Hispanic American follow-up samples, p = 0.001), weight (p = 0.001), and hip circumference (p = 0.0005). We did not replicate association between rs6602024 and obesity-related traits in the GenNet sample, although we found that in European Americans, Hispanic Americans, and African Americans, homozygotes for the rare "A" allele were, on average, 1.0-3.0 BMI units heavier than homozygotes for the more common "G" allele. In summary, we have completed a whole genome-association scan for three obesity-related quantitative traits and report that common genetic variants in the FTO gene are associated with substantial changes in BMI, hip circumference, and body weight. These changes could have a significant impact on the risk of obesity-related morbidity in the general population.
Objective: Measurement of anthropometric traits including hip circumference, weight, height, and BMI calculation in human subjects across multiple ethnic groups
This is a Anthropometric trait measurement protocol using human as the model organism. The procedure involves 7 procedural steps, 3 equipment items, 1 materials. Extracted from a 2007 paper published in PLoS Genetics.
Model and subjects
human • N/A • unknown • 14-102 years • not specified • 6148
Study window
Estimated timing pending
Core workflow
Subject recruitment and enrollment • Physical examination and anthropometric measurement • Blood sample collection
Primary readouts
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
Use the page like this
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Recruited 6,148 individuals of both sexes, ages 14-102 years from a cluster of four towns in the Lanusei Valley
Note: For follow-up GenNet study, 3,467 individuals were recruited between 1995 and 2004 from two field centers
“We recruited and phenotyped 6,148 individuals, male and female, ages 14-102 y, from a cluster of four towns in the Lanusei Valley”
During physical examination of each individual, anthropometric traits were recorded including hip circumference, weight, and height
Note: Measurements performed during same visit as blood collection
“During physical examination of each individual, a blood sample was collected (for DNA extraction) and anthropometric traits were recorded”
Collected blood sample from each individual for DNA extraction
Note: Performed during same physical examination as anthropometric measurements
“During physical examination of each individual, a blood sample was collected (for DNA extraction)”
Calculated BMI as a derived quantity from height and weight measurements
Note: BMI is derived from combination of height and weight measurements
“anthropometric traits were recorded. Here, we report analyses of hip circumference, weight, and the derived quantity BMI (which is calculated from a combination of height and weight)”
DNA extracted from collected blood samples for genotyping
Note: Standard protocols used
“a blood sample was collected (for DNA extraction)”
Genotyping carried out using Affymetrix 10K and 500K chips using standard protocols
Note: Standard protocols applied; quality assessments provided in Results section and Table S1
“Genotyping was carried out using the Affymetrix 10K and 500K chips using standard protocols”
SNP genotyping performed using 5'-nuclease-based TaqMan assay on ABI Prism 7900 Real Time PCR System for follow-up SNPs rs9930506 and rs6602024
Note: Genotype completeness rates exceeded 98% within each ethnic group; no deviation from Hardy-Weinberg equilibrium (p > 0.05)
“SNP genotyping was performed using the 5'-nuclease-based assay (TaqMan; ABI) analyzed on an ABI Prism 7900 Real Time PCR System”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
Measurement of anthropometric traits including hip circumference, weight, height, and BMI calculation in human subjects across multiple ethnic groups
Objective
Measurement of anthropometric traits including hip circumference, weight, height, and BMI calculation in human subjects across multiple ethnic groups
Subjects
From paperhuman • N/A • unknown • 14-102 years • not specified
Sample count
From paper6148
Cohort notes
From paperRecruited from Lanusei Valley cluster of four towns; follow-up GenNet study comprised 3,467 individuals (1,101 AA, 839 HA, 1,496 EA) recruited 1995-2004 from Tecumseh Michigan and Maywood Illinois
Subject recruitment and enrollment (not specified)
Physical examination and anthropometric measurement (not specified)
Blood sample collection (not specified)
BMI calculation (not specified)
Hip circumference (cm)
From paperGenotype data quality assessed with summary assessments provided in Results section and Table S1; Hardy-Weinberg equilibrium tested (p > 0.05); genotype completeness rates calculated within each ethnic group (>98%); association analyses performed between SNPs and anthropometric traits
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Body weight (kg)
From paperGenotype data quality assessed with summary assessments provided in Results section and Table S1; Hardy-Weinberg equilibrium tested (p > 0.05); genotype completeness rates calculated within each ethnic group (>98%); association analyses performed between SNPs and anthropometric traits
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Height (cm)
From paperGenotype data quality assessed with summary assessments provided in Results section and Table S1; Hardy-Weinberg equilibrium tested (p > 0.05); genotype completeness rates calculated within each ethnic group (>98%); association analyses performed between SNPs and anthropometric traits
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
BMI (kg/m²)
From paperGenotype data quality assessed with summary assessments provided in Results section and Table S1; Hardy-Weinberg equilibrium tested (p > 0.05); genotype completeness rates calculated within each ethnic group (>98%); association analyses performed between SNPs and anthropometric traits
Artifact type
Endpoint measurements summarized by group or timepoint
Comparison focus
Compare endpoint magnitude between groups, timepoints, or both
Hip circumference (cm)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Body weight (kg)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Height (cm)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
BMI (kg/m²)
From paperRaw artifact
Per-sample or per-animal endpoint measurements collected during the experiment
Processed artifact
Structured table with cleaned measurements ready for comparison
Final reported form
Summary statistics and between-group or across-timepoint comparisons
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
Preprocessing / cleaning
Genotype data quality assessed with summary assessments provided in Results section and Table S1; Hardy-Weinberg equilibrium tested (p > 0.05); genotype completeness rates calculated within each ethnic group (>98%); association analyses performed between SNPs and anthropometric traits
Scoring or quantification
Quantify the primary readouts for this experiment: Hip circumference (cm); Body weight (kg); Height (cm); BMI (kg/m²).
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Hip circumference (cm), Body weight (kg), Height (cm), BMI (kg/m²).
Source links and direct wording from the methods section for validation and deeper review.
Citation
Angelo Scuteri et al. (2007). Genome-Wide Association Scan Shows Genetic Variants in the FTO Gene Are Associated with Obesity-Related Traits. PLoS Genetics
“”
“”
“”
“”
Direct vendor pages are linked from the protocol above. This section stays focused on the full comparison view and the prep checklist.
Gather these items before starting the experiment. Check off items as you prepare.
Affymetrix • 10K • not specified • N/A
Affymetrix • 500K • not specified • N/A
Applied Biosystems (ABI) • Prism 7900 • not specified • N/A
not specified • not specified • not specified • N/A
Applied Biosystems (ABI) • N/A
Use this section as the page quality checkpoint. It keeps section navigation, evidence access, readiness, and verification meaning in one place.
Current status surfaces were computed from experiment data updated Feb 28, 2026.
Source access
Jump back into the original paper or the methods evidence section when you need exact wording, exclusions, or method-specific caveats.
This protocol has structured steps plus evidence quotes, and is ready for canonical sync.
Steps
7
Evidence Quotes
11
Protocol Items
4
Linked Products
0
Canonical Sync
Pending
What this means
The completeness score reflects how much structured protocol data is present: steps, methods evidence, listed materials, linked products, and paper provenance.
Computed from the current experiment record updated Feb 28, 2026.
Canonical Sync shows whether a ConductGraph-backed protocol is available for this experiment route right now. It is a sync-status signal, not a claim that every downstream vendor link or step detail is perfect.
Steps
7
Evidence
11
Specific Products
0/0
Canonical Sync
Pending
What this score means
The verification score reflects evidence coverage, subject detail, paper provenance, step depth, and whether linked products resolve to specific item pages instead of generic searches.
Computed from the current experiment record updated Feb 28, 2026.
A page can have structured steps and still need review when evidence is thin, product links are generic, or canonical protocol coverage is still pending.
What still needs work
Music Experience Rating Scale
Interpretative Phenomenological Analysis
Semi-structured Interviews on Music Experience in Psilocybin Therapy
Nicotine Patch Challenge Study