Nicotine Patch Challenge Study
Objective: To identify cingulate functional circuits associated with nicotine addiction severity and to study how acute nicotine administration affects these functional pathways using resting-state fMRI
This is a Nicotine Patch Challenge Study protocol using human as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 2 materials. Extracted from a 2009 paper published in Archives of General Psychiatry.
Model and subjects
human • N/A • unknown • Not specified • Not specified • 19
Study window
Estimated timing pending
Core workflow
Study design setup • Baseline resting-state fMRI acquisition • Nicotine or placebo patch administration
Primary readouts
- Correlation between nicotine addiction severity and dorsal anterior cingulate cortex (dACC)-striatal functional connectivity strength
- Effects of acute nicotine patch administration on cingulate-neocortical functional connectivity patterns
- Resting-state functional connectivity changes in response to nicotine versus placebo
- Identification of separate functional circuits associated with nicotine addiction versus nicotinic action
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Study design setup
Implement double-blind, placebo-controlled study design with participants randomized to receive either nicotine patch or placebo patch
Note: Double-blind design ensures neither participants nor researchers know treatment assignment
View evidence from paper
“double-blind, placebo-controlled design”
Baseline resting-state fMRI acquisition
Acquire resting-state fMRI signals from cingulate cortex region to establish baseline functional connectivity patterns
Note: Region-specific signals used to extract cingulate functional connectivity
View evidence from paper
“Using region-specific resting-state fMRI signals to extract resting-state cingulate functional connectivity”
Nicotine or placebo patch administration
Apply single dose nicotine patch (21/35mg) or placebo patch to participant
Note: Single dose administration in double-blind fashion
View evidence from paper
“Single dose (21/35mg) nicotine patch”
Post-administration resting-state fMRI acquisition
Acquire resting-state fMRI signals following patch administration to measure acute effects on functional connectivity
Note: Timing of post-administration scan not specified in text
View evidence from paper
“study how nicotine addiction and acute nicotine administration modulate these functional pathways”
Functional connectivity analysis
Analyze correlation between nicotine addiction severity and cingulate resting-state functional connectivity patterns, comparing baseline and post-nicotine conditions
Note: Separate pathways identified for addiction severity versus acute nicotinic action
View evidence from paper
“Correlation of nicotine addiction severity and cingulate resting state functional connectivity, and effects of acute nicotine on connectivity strength”