Source Paper
Manon Ranger, Cecil M. Y. Chau, Amanmeet Garg, Todd S. Woodward, Mirza Faisal Beg et al.
PLoS ONE • 2013
In very preterm children without major sensory, motor or cognitive impairments, neonatal pain-related stress appears to be associated with thinner cortex in multiple regions at school age, independent of other neonatal risk factors.
Objective: Examine the association between neonatal pain-related stress (skin-breaking procedures) and cortical thickness in very preterm children using constrained principal component analysis and generalized linear modeling
This is a Neonatal Pain-Related Stress Association Analysis protocol using human as the model organism. The procedure involves 7 procedural steps, 1 equipment items. Extracted from a 2013 paper published in PLoS ONE.
Model and subjects
human • mean age 7.9 years at neuroimaging • 42
Study window
~32 week study window
Core workflow
Subject recruitment and longitudinal follow-up • MRI neuroimaging acquisition • Brain segmentation and cortical thickness calculation
Primary readouts
Key equipment and reagents
Verified items
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Direct vendor links
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Enroll 42 right-handed children born very preterm (24-32 weeks gestational age) and follow them longitudinally from birth
Note: Exclude children with severe brain injury and major motor/sensory/cognitive impairment
“42 right-handed children born very preterm (24–32 weeks gestational age) followed longitudinally from birth”
Perform 3-D T1 MRI neuroimaging at mean age 7.9 years
“underwent 3-D T1 MRI neuroimaging at mean age 7.9 yrs”
Process MRI data using FreeSurfer segmentation and custom developed software to calculate regional cortical thickness
“Regional cortical thickness was calculated using custom developed software utilizing FreeSurfer segmentation data”
Define neonatal pain-related stress as the number of skin-breaking procedures during neonatal period
“neonatal pain-related stress (defined as the number of skin-breaking procedures)”
Record clinical confounders including gestational age, illness severity, infection, mechanical ventilation, surgeries, and morphine exposure
“accounting for clinical confounders (gestational age, illness severity, infection, mechanical ventilation, surgeries, and morphine exposure)”
Apply constrained principal component analysis to examine the association between neonatal pain-related stress and cortical thickness while accounting for clinical confounders
“was examined in relation to cortical thickness using constrained principal component analysis followed by generalized linear modeling”
Perform generalized linear modeling to further analyze the association between neonatal pain-related stress and cortical thickness
“constrained principal component analysis followed by generalized linear modeling”
This section explains what the experiment is doing, which readouts matter, what the data artifacts usually look like, and how the analysis should flow from raw capture to reported result.
Examine the association between neonatal pain-related stress (skin-breaking procedures) and cortical thickness in very preterm children using constrained principal component analysis and generalized linear modeling
Objective
Examine the association between neonatal pain-related stress (skin-breaking procedures) and cortical thickness in very preterm children using constrained principal component analysis and generalized linear modeling
Subjects
From paperhuman • mean age 7.9 years at neuroimaging
Sample count
From paper42
Cohort notes
From paperRight-handed children born very preterm (24-32 weeks gestational age), followed longitudinally from birth.
Subject recruitment and longitudinal follow-up
MRI neuroimaging acquisition (At mean age 7.9 years)
Brain segmentation and cortical thickness calculation
Quantify neonatal pain-related stress exposure
Regional cortical thickness
From paperConstrained principal component analysis followed by generalized linear modeling, accounting for clinical confounders (gestational age, illness severity, infection, mechanical ventilation, surgeries, and morphine exposure)
Artifact type
Representative image panels with region or marker comparisons
Comparison focus
Compare staining intensity, structure, or cell counts across matched conditions
Association between neonatal pain-related stress (number of skin-breaking procedures) and cortical thickness
From paperConstrained principal component analysis followed by generalized linear modeling, accounting for clinical confounders (gestational age, illness severity, infection, mechanical ventilation, surgeries, and morphine exposure)
Artifact type
Representative image panels with region or marker comparisons
Comparison focus
Compare staining intensity, structure, or cell counts across matched conditions
Regional cortical thickness
From paperRaw artifact
Field or section images captured from matched samples
Processed artifact
Selected representative panels with quantified intensity, counts, or area measurements
Final reported form
Per-group imaging summaries with representative figures and quantified endpoints
Association between neonatal pain-related stress (number of skin-breaking procedures) and cortical thickness
From paperRaw artifact
Field or section images captured from matched samples
Processed artifact
Selected representative panels with quantified intensity, counts, or area measurements
Final reported form
Per-group imaging summaries with representative figures and quantified endpoints
Acquisition
Capture matched images from the relevant tissue region using the same acquisition settings across samples.
Preprocessing / cleaning
Constrained principal component analysis followed by generalized linear modeling, accounting for clinical confounders (gestational age, illness severity, infection, mechanical ventilation, surgeries, and morphine exposure)
Scoring or quantification
Quantify the primary readouts for this experiment: Regional cortical thickness; Association between neonatal pain-related stress (number of skin-breaking procedures) and cortical thickness.
Normalization
Normalize image-derived measurements against the matched acquisition or segmentation rules before comparing groups.
Statistical comparison
Statistical method not yet structured for this page.
Reporting output
Report representative outputs alongside summary comparisons for Regional cortical thickness, Association between neonatal pain-related stress (number of skin-breaking procedures) and cortical thickness.
Source links and direct wording from the methods section for validation and deeper review.
Citation
Manon Ranger et al. (2013). Neonatal Pain-Related Stress Predicts Cortical Thickness at Age 7 Years in Children Born Very Preterm. PLoS ONE
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Source access
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Steps
7
Evidence Quotes
8
Protocol Items
1
Linked Products
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Canonical Sync
Pending
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Steps
7
Evidence
8
Specific Products
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Canonical Sync
Pending
What this score means
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Computed from the current experiment record updated Feb 28, 2026.
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