Anxiolytic and Antidepressant Behavioral Testing
Objective: Evaluation of anxiolytic and antidepressant-like effects of chronic HU210 treatment in adult rats, and investigation of the correlation between hippocampal neurogenesis and behavioral changes
This is a Anxiolytic and Antidepressant Behavioral Testing protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 materials. Extracted from a 2005 paper published in Journal of Clinical Investigation.
Model and subjects
rat • Not specified • unknown • adult • Not specified
Study window
Estimated timing pending
Core workflow
Chronic HU210 Treatment • Behavioral Testing for Anxiolytic Effects • Behavioral Testing for Antidepressant Effects
Primary readouts
- Anxiolytic-like behavioral effects
- Antidepressant-like behavioral effects
- Hippocampal neurogenesis promotion in the dentate gyrus
- Correlation between neurogenesis and behavioral changes
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Chronic HU210 Treatment
Administer chronic HU210 treatment to adult rats
Note: Chronic, but not acute, HU210 treatment was used. Acute treatment did not produce the same effects.
View evidence from paper
“Chronic, but not acute, HU210 treatment promoted neurogenesis in the hippocampal dentate gyrus of adult rats”
Behavioral Testing for Anxiolytic Effects
Evaluate anxiolytic-like effects following chronic HU210 treatment
Note: Specific behavioral tests not detailed in provided text
View evidence from paper
“exerted anxiolytic- and antidepressant-like effects”
Behavioral Testing for Antidepressant Effects
Evaluate antidepressant-like effects following chronic HU210 treatment
Note: Specific behavioral tests not detailed in provided text
View evidence from paper
“exerted anxiolytic- and antidepressant-like effects”
Hippocampal X-irradiation Control
X-irradiate the hippocampus in control animals to block neurogenesis and assess its necessity for behavioral effects
Note: X-irradiation blocked both neurogenic and behavioral effects of chronic HU210 treatment
View evidence from paper
“X-irradiation of the hippocampus blocked both the neurogenic and behavioral effects of chronic HU210 treatment”