Source Paper
Memory Consolidation for Contextual and Auditory Fear Conditioning Is Dependent on Protein Synthesis, PKA, and MAP Kinase
Glenn E. Schafe, Nicole V. Nadel, Gregory M. Sullivan, Alexander Harris, Joseph E. LeDoux
Learning & Memory • 1999
Auditory Fear Conditioning
Objective: Examine whether inhibition of protein synthesis, PKA, and MAP kinase activity interferes with consolidation of contextual and auditory fear conditioning memory in rats
This is a Auditory Fear Conditioning protocol using rat as the model organism. The procedure involves 4 procedural steps, 3 materials. Extracted from a 1999 paper published in Learning & Memory.
Model and subjects
rat • not specified • unknown • not specified • not specified
Study window
~1 day study window | ~24 hours hands-on
Core workflow
Intraventricular injection of inhibitors • Fear conditioning • Short-term memory assessment
Primary readouts
- Retention of contextual fear memory at short-term (within 1 hour)
- Retention of auditory fear memory at short-term (within 1 hour)
- Retention of contextual fear memory at long-term (24 hours)
- Retention of auditory fear memory at long-term (24 hours)
Key equipment and reagents
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Protocol Steps
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Intraventricular injection of inhibitors
Rats received intraventricular injections of protein synthesis, PKA, or MAP kinase inhibitors prior to fear conditioning
Note: Three different compounds tested at two dose levels each: Anisomycin (100 or 300 µg), Rp-cAMPS (90 or 180 µg), or PD098059 (1 or 3 µg)
View evidence from paper
“Rats were injected intraventricularly with Anisomycin (100 or 300 µg), Rp-cAMPS (90 or 180 µg), or PD098059 (1 or 3 µg) prior to conditioning”
Fear conditioning
Rats underwent contextual and auditory fear conditioning following drug injection
Note: Specific conditioning parameters not detailed in provided text
View evidence from paper
“prior to conditioning and assessed for retention of contextual and auditory fear memory”
Short-term memory assessment
Assessment of contextual and auditory fear memory retention within one hour of conditioning
Note: Short-term memory was found to be intact despite drug treatment
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”
Long-term memory assessment
Assessment of contextual and auditory fear memory retention 24 hours after conditioning
Note: Long-term memory was selectively impaired by all three inhibitors
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”