Source Paper
Memory Consolidation for Contextual and Auditory Fear Conditioning Is Dependent on Protein Synthesis, PKA, and MAP Kinase
Glenn E. Schafe, Nicole V. Nadel, Gregory M. Sullivan, Alexander Harris, Joseph E. LeDoux
Learning & Memory • 1999
Auditory Fear Conditioning
Objective: Examine whether inhibition of protein synthesis, PKA, and MAP kinase activity interferes with consolidation of contextual and auditory fear conditioning memory in rats
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Protocol Steps
Intraventricular injection of inhibitors
Rats received intraventricular injections of protein synthesis, PKA, or MAP kinase inhibitors prior to fear conditioning
Note: Three different compounds tested at two dose levels each: Anisomycin (100 or 300 μg), Rp-cAMPS (90 or 180 μg), or PD098059 (1 or 3 μg)
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“Rats were injected intraventricularly with Anisomycin (100 or 300 μg), Rp-cAMPS (90 or 180 μg), or PD098059 (1 or 3 μg) prior to conditioning”
Fear conditioning
Rats underwent contextual and auditory fear conditioning following drug injection
Note: Specific conditioning parameters not detailed in provided text
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“prior to conditioning and assessed for retention of contextual and auditory fear memory”
Short-term memory assessment
Assessment of contextual and auditory fear memory retention within one hour of conditioning
Note: Short-term memory was found to be intact despite drug treatment
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“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”
Long-term memory assessment
Assessment of contextual and auditory fear memory retention 24 hours after conditioning
Note: Long-term memory was selectively impaired by all three inhibitors
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”