Source Paper
Memory for Extinction of Conditioned Fear Is Long-lasting and Persists Following Spontaneous Recovery
Learning & Memory • 2002
Auditory Fear Conditioning
Objective: To examine the persistence of extinction memory following auditory fear conditioning and spontaneous recovery, and to determine whether extinction training interferes with conditioning consolidation when given on the same day
This is a Auditory Fear Conditioning protocol using rat as the model organism. The procedure involves 5 procedural steps, 1 equipment items. Extracted from a 2002 paper published in Learning & Memory.
Model and subjects
rat • not specified • unknown • not specified • not specified
Study window
~2 week study window | ~1 hours hands-on
Core workflow
Auditory Fear Conditioning • Extinction Training • Spontaneous Recovery Testing
Primary readouts
- Conditioned freezing response to tone
- Spontaneous recovery of freezing over time
- Rate of re-extinction
- Persistence of extinction memory
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Auditory Fear Conditioning
Rats received 7 trials of auditory fear conditioning in which a tone was paired with footshock
Note: Initial conditioning phase to establish conditioned fear response
View evidence from paper
“we gave rats 7 trials of auditory fear conditioning followed 1 h later by 20 extinction trials”
Extinction Training
Rats received 20 extinction trials where the tone was presented in the absence of footshock
Note: Extinction training was conducted 1 hour following the completion of conditioning trials
View evidence from paper
“we gave rats 7 trials of auditory fear conditioning followed 1 h later by 20 extinction trials”
Spontaneous Recovery Testing
Rats were tested for spontaneous recovery of conditioned freezing to the tone at various time delays following extinction training
Note: Multiple test timepoints to assess recovery of conditioned fear response over time
View evidence from paper
“tested for spontaneous recovery after a delay of 0, 1, 2, 4, 6, 10, or 14 d”
No-Extinction Control Testing
Control rats that did not receive extinction training were tested to determine if increases in freezing were due to incubation of conditioning memory
Note: Control condition to distinguish between spontaneous recovery and incubation effects
View evidence from paper
“No-extinction controls indicated that the increase in freezing with time was not owing to incubation of conditioning memory”
Re-extinction Testing
Rats were assessed for savings in their rate of re-extinction following spontaneous recovery
Note: Measures persistence of extinction memory despite complete recovery of freezing
View evidence from paper
“rats showed savings in their rate of re-extinction, indicating persistence of extinction memory”