Source Paper
Reactivation of Hippocampal Cell Assemblies: Effects of Behavioral State, Experience, and EEG Dynamics
Hemant S. Kudrimoti, Carol A. Barnes, Bruce L. McNaughton
Journal of Neuroscience • 1999
Behavioral State Tracking
Objective: Classification and analysis of behavioral states including quiet wakefulness, slow wave sleep (SWS), and rapid eye movement (REM) sleep, and study of spontaneous reactivation of neuronal activity patterns across different behavioral states
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Protocol Steps
Behavioral state identification
Classify and identify three behavioral states: quiet wakefulness, slow wave sleep (SWS), and rapid eye movement (REM) sleep
Note: States were distinguished based on EEG patterns and behavioral observations
View evidence from paper
“The effects of behavioral state [quiet wakefulness, SWS, and rapid eye movement (REM)]”
Simultaneous neuronal recording during behavioral states
Record pairwise firing-rate correlations within populations of simultaneously recorded CA1 pyramidal neurons across different behavioral states
Note: Recordings were made during quiet wakefulness, SWS, and REM sleep
View evidence from paper
“pairwise firing-rate correlations within a population of simultaneously recorded neurons”
Behavioral experience exposure
Expose animals to spatial experiences and record neuronal activity patterns during these experiences
Note: Two successive spatial experiences were tested, including both novel and repeated experiences
View evidence from paper
“interactions between two successive spatial experiences”
Post-experience sleep recording
Record neuronal activity patterns during SWS and quiet wakefulness following behavioral experiences
Note: Recordings were made in subsequent SWS or quiet wakefulness after experiences
View evidence from paper
“Pairwise firing-rate correlations during often repeated experiences accounted for a significant proportion of the variance in these interactions in subsequent SWS or quiet wakefulness”
Sharp wave-ripple oscillation analysis
Identify and analyze neuronal activity patterns during sharp wave-ripple oscillations (200 Hz EEG ripples)
Note: Pattern reinstatement was strongest during these oscillations
View evidence from paper
“Pattern reinstatement was strongest during sharp wave–ripple oscillations”
REM sleep analysis
Analyze firing-rate correlations during REM sleep episodes and their relationship to preceding experiences
Note: REM sleep correlations were not related to preceding familiar experiences
View evidence from paper
“Firing-rate correlations during REM sleep were not related to the preceding familiar experience”