Behavioral Stress Response Testing
Objective: Assessment of behavioral responses to stress following methionine infusion and epigenetic modification, examining whether epigenetic programming of stress responses is reversible in adult life
This is a Behavioral Stress Response Testing protocol using rat as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 1 materials. Extracted from a 2005 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified in provided text • unknown • adult • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Central methionine infusion • Behavioral stress response assessment
Primary readouts
- DNA methylation patterns in hippocampal exon 1_7 glucocorticoid receptor promoter
- Nerve growth factor-inducible protein-A binding to exon 1_7 promoter
- Glucocorticoid receptor (GR) expression
- Hypothalamic-pituitary-adrenal (HPA) axis responses to stress
Key equipment and reagents
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Protocol Steps
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Central methionine infusion
Adult offspring received central infusion of L-methionine, an essential amino acid precursor to S-adenosyl-methionine that serves as methyl group donor for DNA methylation
Note: Infusion was performed in adult offspring to examine reversibility of epigenetic programming
View evidence from paper
“centrally infused the adult offspring with the essential amino acid l-methionine, a precursor to S-adenosyl-methionine that serves as the donor of methyl groups for DNA methylation”
Behavioral stress response assessment
Behavioral responses to stress were measured following methionine infusion
Note: Stress responses were assessed as outcome measure of epigenetic modification
View evidence from paper
“behavioral responses to stress, suggesting a causal relationship among epigenomic state, GR expression, and stress responses in the adult offspring”