Behavioral Stress Response Testing
Objective: Assessment of behavioral responses to stress following methionine infusion and epigenetic modification, examining whether epigenetic programming of stress responses is reversible in adult life
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified in provided text • Not specified • Not specified • Not mentioned
Materials1
Not specified • Not applicable • Not specified • Not mentioned
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Protocol Steps
Central methionine infusion
Adult offspring received central infusion of L-methionine, an essential amino acid precursor to S-adenosyl-methionine that serves as methyl group donor for DNA methylation
Note: Infusion was performed in adult offspring to examine reversibility of epigenetic programming
View evidence from paper
“centrally infused the adult offspring with the essential amino acid l-methionine, a precursor to S-adenosyl-methionine that serves as the donor of methyl groups for DNA methylation”
Behavioral stress response assessment
Behavioral responses to stress were measured following methionine infusion
Note: Stress responses were assessed as outcome measure of epigenetic modification
View evidence from paper
“behavioral responses to stress, suggesting a causal relationship among epigenomic state, GR expression, and stress responses in the adult offspring”