Bilateral Lesion Study
Objective: Examine the role of the hippocampus in recognition memory using bilateral cytotoxic lesions in hippocampus, perirhinal cortex, or prefrontal cortex, and test recognition memory judgments using different types of mnemonic information including object familiarity, spatial location, and temporal recency
This is a Bilateral Lesion Study protocol using rat as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 1 materials. Extracted from a 2011 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Surgical preparation - Bilateral lesions • Surgical preparation - Unilateral combined lesions • Novel object preference task
Primary readouts
- Novel object preference performance
- Object-in-place memory performance
- Temporal order memory performance
- Recognition memory judgments using different types of mnemonic information
Key equipment and reagents
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Protocol Steps
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Surgical preparation - Bilateral lesions
Rats were prepared with bilateral cytotoxic lesions in the hippocampus, perirhinal cortex, or prefrontal cortex
Note: Experiment 1 design: separate groups received lesions in different brain regions
View evidence from paper
“Rats with bilateral cytotoxic lesions in the hippocampus or perirhinal or prefrontal cortex”
Surgical preparation - Unilateral combined lesions
Groups of rats were prepared with a unilateral cytotoxic lesion in the hippocampus combined with a lesion in either the contralateral perirhinal or prefrontal cortex
Note: Experiment 2 design: unilateral hippocampal lesion paired with contralateral cortical lesion
View evidence from paper
“groups of rats were prepared with a unilateral cytotoxic lesion in the hippocampus combined with a lesion in either the contralateral perirhinal or prefrontal cortex”
Novel object preference task
Rats tested on recognition memory task requiring animals to make recognition memory judgments using familiarity (novel object preference)
Note: Tests object familiarity discrimination
View evidence from paper
“requiring the animals to make recognition memory judgments using familiarity (novel object preference)”
Object-in-place memory task
Rats tested on recognition memory task requiring animals to use object-place information (object-in-place memory)
Note: Tests spatial location memory for objects
View evidence from paper
“object–place information (object-in-place memory)”
Temporal order memory task
Rats tested on recognition memory task requiring animals to use recency information (temporal order memory)
Note: Tests temporal or object recency component of memory
View evidence from paper
“recency information (temporal order memory)”
Series of object recognition memory tasks
Rats tested in a series of object recognition memory tasks to examine hippocampal interactions with perirhinal or prefrontal cortex
Note: Experiment 2: tests functional interactions between brain regions
View evidence from paper
“Rats were then tested in a series of object recognition memory tasks”