Source Paper
Regulation of BDNF and trkB mRNA in rat brain by chronic electroconvulsive seizure and antidepressant drug treatments
M Nibuya, S Morinobu, RS Duman
Journal of Neuroscience • 1995
Chronic Electroconvulsive Seizure Treatment
Objective: Examine the influence of chronic electroconvulsive seizure (ECS) treatment on BDNF and trkB mRNA expression in rat brain using in situ hybridization and Northern blot analysis
This is a Chronic Electroconvulsive Seizure Treatment protocol using rat as the model organism. The procedure involves 6 procedural steps, 2 equipment items. Extracted from a 1995 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~1.4 week study window
Core workflow
Chronic ECS Treatment Protocol • Acute ECS Administration • Brain Tissue Collection - Early Timepoint
Primary readouts
- BDNF mRNA expression levels in frontal cortex
- BDNF mRNA expression levels in hippocampus (dentate gyrus, CA3, CA1)
- BDNF mRNA expression levels in piriform cortex layer 2
- trkB mRNA expression levels in hippocampus
Key equipment and reagents
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Protocol Steps
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Chronic ECS Treatment Protocol
Administer chronic electroconvulsive seizure treatment over 10 days
Note: Chronic ECS treatment period prior to acute ECS administration
View evidence from paper
“an effect significantly augmented by a prior course of chronic ECS treatment (10 d)”
Acute ECS Administration
Administer acute electroconvulsive seizure treatment following chronic treatment course
Note: Applied after completion of 10-day chronic ECS treatment
View evidence from paper
“In frontal cortex, acute ECS increased BDNF mRNA approximately twofold”
Brain Tissue Collection - Early Timepoint
Collect brain tissue for analysis of BDNF and trkB mRNA expression
Note: Tissue collected to measure acute induction of BDNF and trkB mRNA
View evidence from paper
“In the dentate gyrus granule cell layer, chronic ECS decreased the acute induction of BDNF and trkB mRNA”
Brain Tissue Collection - Delayed Timepoint
Collect brain tissue 18 hours after the last chronic ECS treatment
Note: Measures prolonged expression of BDNF and trkB mRNA following chronic ECS
View evidence from paper
“levels remained elevated two- to threefold 18 hr later after the last chronic ECS treatment, but returned to control 18 hr after acute ECS”
In Situ Hybridization Analysis
Perform in situ hybridization on brain tissue sections to visualize and quantify BDNF and trkB mRNA expression in specific brain regions
Note: Analyze frontal cortex, hippocampus (dentate gyrus, CA3, CA1), and piriform cortex layer 2
View evidence from paper
“expression of brain-derived neurotrophic factor (BDNF) and its receptor, trkB, was examined by in situ hybridization and Northern blot”
Northern Blot Analysis
Perform Northern blot analysis on brain tissue to quantify BDNF and trkB mRNA levels
Note: Provides quantitative measurement of mRNA expression levels
View evidence from paper
“expression of brain-derived neurotrophic factor (BDNF) and its receptor, trkB, was examined by in situ hybridization and Northern blot”