Chronic Immobilization Stress
Objective: To examine the effects of chronic immobilization stress on hippocampal and amygdaloid neuronal morphology in rats, specifically measuring dendritic remodeling patterns and behavioral changes in anxiety-like responses
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Equipment1
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Protocol Steps
Chronic Immobilization Stress (CIS) Application
Apply chronic immobilization stress to rats as one of two chronic stress models examined in the study
Note: CIS is one of two stress models tested; the other is Chronic Unpredictable Stress (CUS)
View evidence from paper
“we examined the effects of two different models of chronic stress on hippocampal and amygdaloid neuronal morphology in rats”
Behavioral Testing - Elevated Plus-Maze
Assess open-arm activity in the elevated plus-maze to measure anxiety-like behavioral responses following chronic immobilization stress
Note: CIS reduced open-arm activity compared to control; CUS had minimal effect on this measure
View evidence from paper
“CIS, but not CUS, reduced open-arm activity in the elevated plus-maze”
Neuronal Morphology Analysis
Examine dendritic remodeling patterns in hippocampal CA3 pyramidal neurons and amygdaloid neurons (pyramidal, stellate, and bipolar neurons in basolateral complex)
Note: CIS induced dendritic atrophy and debranching in CA3 pyramidal neurons but enhanced dendritic arborization in amygdala pyramidal and stellate neurons
View evidence from paper
“chronic immobilization stress (CIS) induced dendritic atrophy and debranching in CA3 pyramidal neurons of the hippocampus. In striking contrast, pyramidal and stellate neurons in the basolateral complex of the amygdala exhibited enhanced dendritic arborization”