Source Paper
Dopamine Release in the Dorsal Striatum during Cocaine-Seeking Behavior under the Control of a Drug-Associated Cue
Rutsuko Ito, Jeffrey W. Dalley, Trevor W. Robbins, Barry J. Everitt
Journal of Neuroscience • 2002
Cocaine Self-Administration with Second-Order Schedule
Objective: Investigate the role of dorsal striatal dopamine innervation in cocaine-seeking behavior under the control of a drug-associated cue using in vivo microdialysis combined with a second-order schedule of cocaine reinforcement
This is a Cocaine Self-Administration with Second-Order Schedule protocol using rat as the model organism. The procedure involves 4 procedural steps, 4 equipment items, 1 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Initial cocaine self-administration training under continuous reinforcement • Progressive increase in response requirement • Microdialysis session with contingent cue presentation
Primary readouts
- Dopamine release in the dorsal striatum measured via microdialysis
- Lever responding during cocaine-seeking behavior
- Differential dopamine response to contingent versus noncontingent cue presentation
Key equipment and reagents
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Protocol Steps
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Initial cocaine self-administration training under continuous reinforcement
Rats were trained to self-administer cocaine under a continuous reinforcement schedule where a single response on one of two identical levers resulted in both a light cue presentation and cocaine infusion
Note: Continuous reinforcement schedule used as initial training phase
View evidence from paper
“Rats were initially trained to self-administer cocaine under a continuous reinforcement schedule where a response on one of two identical levers led to a 20 sec presentation of a light CS+ and an intravenous cocaine infusion (0.75 mg/kg)”
Progressive increase in response requirement
The response requirement for obtaining the CS+ and cocaine was progressively increased until stable responding was established under a second-order schedule of reinforcement
Note: Response requirement increased gradually to establish second-order schedule
View evidence from paper
“The response requirement for the CS+ and cocaine was then progressively increased until stable responding was established under a second-order schedule of reinforcement”
Microdialysis session with contingent cue presentation
During microdialysis recording, rats were presented with the cocaine-associated light cue contingent on their responding during a cocaine-seeking behavior session
Note: Contingent presentation means cue was presented only when rat responded on lever
View evidence from paper
“During microdialysis, rats were presented with the cocaine-associated CS+ either noncontingently or contingent on responding during a session of cocaine-seeking behavior”
Microdialysis session with noncontingent cue presentation
During microdialysis recording, rats were presented with the cocaine-associated light cue noncontingently (independent of their responding) during a cocaine-seeking behavior session
Note: Noncontingent presentation means cue was presented regardless of rat behavior
View evidence from paper
“During microdialysis, rats were presented with the cocaine-associated CS+ either noncontingently or contingent on responding during a session of cocaine-seeking behavior”