Comparative Stem Cell Transplantation
Objective: Compare locomotor recovery outcomes between human dental pulp stem cells, bone marrow stromal cells, and skin-derived fibroblasts transplanted into completely transected rat spinal cord
This is a Comparative Stem Cell Transplantation protocol using rat as the model organism. The procedure involves 6 procedural steps, 3 materials. Extracted from a 2011 paper published in Journal of Clinical Investigation.
Model and subjects
rat • adult rat • not specified • adult • not specified • not specified
Study window
Estimated timing pending
Core workflow
Spinal cord transection • Cell transplantation • Assessment of locomotor recovery
Primary readouts
- Hind limb locomotor function recovery
- Preservation of neuronal filaments and myelin sheaths
- Inhibition of SCI-induced apoptosis in neurons, astrocytes, and oligodendrocytes
- Transected axon regeneration
Key equipment and reagents
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Protocol Steps
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Spinal cord transection
Complete transection of the adult rat spinal cord to create the injury model
Note: Complete transection model used for all groups
View evidence from paper
“transplantation of human dental pulp stem cells into the completely transected adult rat spinal cord”
Cell transplantation
Transplantation of one of three cell types (dental pulp stem cells, bone marrow stromal cells, or skin-derived fibroblasts) into the transected spinal cord
Note: Three experimental groups compared: dental pulp stem cells, bone marrow stromal cells, and skin-derived fibroblasts
View evidence from paper
“Transplantation of human dental pulp stem cells into the completely transected adult rat spinal cord resulted in marked recovery of hind limb locomotor functions”
Assessment of locomotor recovery
Evaluation of hind limb locomotor function recovery following cell transplantation
Note: Primary outcome measure was locomotor recovery
View evidence from paper
“resulted in marked recovery of hind limb locomotor functions”
Histological analysis of neuroprotection
Examination of neuronal filaments and myelin sheath preservation to assess inhibition of apoptosis
Note: Assessed preservation of neurons, astrocytes, and oligodendrocytes
View evidence from paper
“they inhibited the SCI-induced apoptosis of neurons, astrocytes, and oligodendrocytes, which improved the preservation of neuronal filaments and myelin sheaths”
Assessment of axon regeneration
Evaluation of transected axon regeneration and inhibition of axon growth inhibitors
Note: Examined inhibition of chondroitin sulfate proteoglycan and myelin-associated glycoprotein
View evidence from paper
“they promoted the regeneration of transected axons by directly inhibiting multiple axon growth inhibitors, including chondroitin sulfate proteoglycan and myelin-associated glycoprotein”
Assessment of cell differentiation
Evaluation of transplanted cell differentiation into mature oligodendrocytes
Note: Assessed cell replacement of lost cells through differentiation
View evidence from paper
“they replaced lost cells by differentiating into mature oligodendrocytes under the extreme conditions of SCI”