Source Paper
Consolidation of Extinction Learning Involves Transfer from NMDA-Independent to NMDA-Dependent Memory
Edwin Santini, Robert U. Muller, Gregory J. Quirk
Journal of Neuroscience • 2001
Conditioned Emotional Response (Bar Pressing Suppression)
Objective: Examine the effects of NMDA antagonist CPP on extinction of conditioned fear responses (freezing and bar pressing suppression) and determine the role of NMDA receptors in consolidation of extinction learning
This is a Conditioned Emotional Response (Bar Pressing Suppression) protocol using rat as the model organism. The procedure involves 8 procedural steps, 2 equipment items, 2 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
rat • not specified • unknown • not specified • not specified
Study window
~2 day study window | ~73.5 hours hands-on
Core workflow
Fear conditioning with tone-shock pairing • Systemic CPP administration • Extinction training session
Primary readouts
- Conditioned freezing response during extinction and recall tests
- Suppression of bar pressing during extinction and recall tests
- Extinction recall at 24 hours post-training
- Extinction recall at 48 hours post-training
Key equipment and reagents
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Protocol Steps
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Fear conditioning with tone-shock pairing
Rats are exposed to a tone paired with foot shock to establish conditioned fear response
Note: This establishes the baseline conditioned emotional response to be measured
View evidence from paper
“Extinction of conditioned fear to a tone paired with foot shock”
Systemic CPP administration
Administer NMDA antagonist CPP systemically at 10 mg/kg dose
Note: CPP is administered prior to extinction session
View evidence from paper
“Rats extinguished normally during a 90 min session in the presence of systemic CPP (10 mg/kg)”
Extinction training session
Expose rats to the conditioned tone in the absence of foot shock to allow extinction learning
Note: Conducted in presence of CPP; rats show normal extinction during this session
View evidence from paper
“Rats extinguished normally during a 90 min session in the presence of systemic CPP (10 mg/kg)”
24-hour recall test
Test extinction recall 24 hours after extinction training session
Note: Rats trained under CPP show impaired recall at 24 hours
View evidence from paper
“were unable to recall extinction learning 24 hr later”
48-hour recall test
Test extinction recall 48 hours after extinction training session
Note: Rats trained under CPP show normal recall at 48 hours, suggesting delayed consolidation
View evidence from paper
“extinction learned in the presence of CPP was recalled normally when tested 48 hr after training”
CPP administration on rest day
Administer CPP on the rest day between extinction training and 48-hour test
Note: Control experiment to test if NMDA receptors are required for delayed consolidation
View evidence from paper
“CPP injected on the rest day between training and test prevented 48 hr recall of extinction learned under CPP”
Measure conditioned freezing response
Quantify freezing behavior as a measure of conditioned emotional response
Note: One of two primary outcome measures alongside bar pressing suppression
View evidence from paper
“extinction of conditioned freezing and suppression of bar pressing (conditioned emotional response)”
Measure bar pressing suppression
Quantify suppression of bar pressing behavior as a measure of conditioned emotional response
Note: One of two primary outcome measures alongside freezing
View evidence from paper
“extinction of conditioned freezing and suppression of bar pressing (conditioned emotional response)”