Conditioned Place Preference
Objective: Assessment of food reward by measuring preference for an environment previously paired with chocolate pellets after Exendin-4 administration to determine the impact of GLP-1 agonist on mesolimbic reward system
This is a Conditioned Place Preference protocol using rat as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 3 materials. Extracted from a 2012 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Conditioned Place Preference Test Setup • Exendin-4 Administration • CPP Test Assessment
Primary readouts
- Preference for environment previously paired with chocolate pellets
- Motivated behavior for sucrose in progressive ratio operant-conditioning
- Presence or absence of malaise
- Locomotor activity/impairment
Key equipment and reagents
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Protocol Steps
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Conditioned Place Preference Test Setup
Rats were exposed to an environment that was paired with chocolate pellets as a food reward
Note: This creates the conditioned association between environment and reward
View evidence from paper
“Food-reward behavior was reduced in the CPP test by EX4, as rats no longer preferred an environment previously paired to chocolate pellets.”
Exendin-4 Administration
EX4 was administered via peripheral, central, or intramesolimbic routes to assess impact on food reward behavior
Note: Multiple administration routes were tested to determine site of action
View evidence from paper
“We assessed the impact of peripheral, central, and intramesolimbic EX4 on two models of food reward: conditioned place preference (CPP) and progressive ratio operant-conditioning.”
CPP Test Assessment
Measurement of rat preference for the environment previously paired with chocolate pellets after EX4 administration
Note: Reduced preference indicates decreased rewarding value of food
View evidence from paper
“Food-reward behavior was reduced in the CPP test by EX4, as rats no longer preferred an environment previously paired to chocolate pellets.”
Progressive Ratio Operant-Conditioning Test
Assessment of motivated behavior for sucrose reward following peripheral EX4 administration
Note: Measures the effort rats will expend to obtain food reward
View evidence from paper
“EX4 also decreased motivated behavior for sucrose in a progressive ratio operant-conditioning paradigm when administered peripherally.”
Mesolimbic Site Identification
Intramesolimbic EX4 administration to ventral tegmental area and nucleus accumbens to determine neurobiological substrates of food-reward suppression
Note: Testing conducted without inducing concurrent malaise or locomotor impairment
View evidence from paper
“We found that the EX4-mediated inhibition of food reward could be driven from two key mesolimbic structures—ventral tegmental area and nucleus accumbens—without inducing concurrent malaise or locomotor impairment.”