Source Paper
Memory Consolidation for Contextual and Auditory Fear Conditioning Is Dependent on Protein Synthesis, PKA, and MAP Kinase
Glenn E. Schafe, Nicole V. Nadel, Gregory M. Sullivan, Alexander Harris, Joseph E. LeDoux
Learning & Memory • 1999
Contextual Fear Conditioning
Objective: Assessment of rats' retention of contextual fear memory following intraventricular injection of protein synthesis, PKA, or MAP kinase inhibitors to examine whether inhibition of these molecular pathways interferes with fear memory consolidation
This is a Contextual Fear Conditioning protocol using rat as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 3 materials. Extracted from a 1999 paper published in Learning & Memory.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~1 day study window | ~24 hours hands-on
Core workflow
Intraventricular drug injection • Fear conditioning • Short-term memory assessment
Primary readouts
- Retention of contextual fear memory at short-term (within 1 hour) and long-term (24 hours) timepoints
- Retention of auditory fear memory at short-term (within 1 hour) and long-term (24 hours) timepoints
- Comparison of long-term versus short-term memory effects
Key equipment and reagents
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Protocol Steps
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Intraventricular drug injection
Rats receive intraventricular injection of one of three compounds: Anisomycin (protein synthesis inhibitor), Rp-cAMPS (PKA inhibitor), or PD098059 (MAP kinase inhibitor) at specified doses
Note: Injections performed prior to conditioning
View evidence from paper
“Rats were injected intraventricularly with Anisomycin (100 or 300 µg), Rp-cAMPS (90 or 180 µg), or PD098059 (1 or 3 µg) prior to conditioning”
Fear conditioning
Rats undergo contextual and auditory fear conditioning in the conditioning apparatus
Note: Conditioning occurs after drug injection
View evidence from paper
“prior to conditioning and assessed for retention of contextual and auditory fear memory”
Short-term memory assessment
Rats are assessed for retention of contextual and auditory fear memory within one hour after conditioning
Note: Short-term memory testing
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”
Long-term memory assessment
Rats are assessed for retention of contextual and auditory fear memory 24 hours after conditioning
Note: Long-term memory testing
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”