Source Paper
Memory Consolidation for Contextual and Auditory Fear Conditioning Is Dependent on Protein Synthesis, PKA, and MAP Kinase
Glenn E. Schafe, Nicole V. Nadel, Gregory M. Sullivan, Alexander Harris, Joseph E. LeDoux
Learning & Memory • 1999
Contextual Fear Conditioning
Objective: Assessment of rats' retention of contextual fear memory following intraventricular injection of protein synthesis, PKA, or MAP kinase inhibitors to examine whether inhibition of these molecular pathways interferes with fear memory consolidation
Gather these items before starting the experiment. Check off items as you prepare.
Equipment2
1 item available from ConductScience
Estimated: $5,900.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Intraventricular drug injection
Rats receive intraventricular injection of one of three compounds: Anisomycin (protein synthesis inhibitor), Rp-cAMPS (PKA inhibitor), or PD098059 (MAP kinase inhibitor) at specified doses
Note: Injections performed prior to conditioning
View evidence from paper
“Rats were injected intraventricularly with Anisomycin (100 or 300 μg), Rp-cAMPS (90 or 180 μg), or PD098059 (1 or 3 μg) prior to conditioning”
Fear conditioning
Rats undergo contextual and auditory fear conditioning in the conditioning apparatus
Note: Conditioning occurs after drug injection
View evidence from paper
“prior to conditioning and assessed for retention of contextual and auditory fear memory”
Short-term memory assessment
Rats are assessed for retention of contextual and auditory fear memory within one hour after conditioning
Note: Short-term memory testing
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”
Long-term memory assessment
Rats are assessed for retention of contextual and auditory fear memory 24 hours after conditioning
Note: Long-term memory testing
View evidence from paper
“assessed for retention of contextual and auditory fear memory both within an hour and 24 hr later”