Source Paper
Temporally Graded Retrograde Amnesia of Contextual Fear after Hippocampal Damage in Rats: Within-Subjects Examination
Stephan G. Anagnostaras, Stephen Maren, Michael S. Fanselow
Journal of Neuroscience • 1999
Contextual Fear Conditioning
Objective: Examine retrograde amnesia of contextual fear produced by dorsal hippocampus lesions in a within-subjects design, comparing remote and recent contextual fear memories
This is a Contextual Fear Conditioning protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items. Extracted from a 1999 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
~7.1 week study window
Core workflow
Remote memory conditioning • Recent memory conditioning • Lesion surgery
Primary readouts
- Remote contextual fear memory (freezing behavior)
- Recent contextual fear memory (freezing behavior)
- Tone freezing (auditory cue-based fear)
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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Remote memory conditioning
Rats received tone-shock pairings in the first context to establish remote memory
Note: This establishes the remote memory condition
View evidence from paper
“Rats were given 10 tone–shock pairings in one context (remote memory)”
Recent memory conditioning
Rats received tone-shock pairings in a distinct context with a different tone 50 days after remote memory conditioning
Note: Different context and different tone from remote memory condition
View evidence from paper
“10 tone–shock pairings in a distinct context (with a different tone) 50 d later (recent memory)”
Lesion surgery
Dorsal hippocampus (DH) electrolytic lesions or sham lesions performed 1 day after recent memory conditioning
Note: Within-subjects design with both DH-lesioned and sham control groups
View evidence from paper
“followed by DH or sham lesions 1 d later”
Fear memory testing
Assessment of contextual fear and tone freezing in both remote and recent contexts
Note: Measured freezing behavior as indicator of fear memory
View evidence from paper
“DH-lesioned rats exhibited no deficit in remote contextual fear, but recent contextual fear memory was severely impaired. They also did not exhibit deficits in tone freezing”