Source Paper
Dopamine Release in the Dorsal Striatum during Cocaine-Seeking Behavior under the Control of a Drug-Associated Cue
Rutsuko Ito, Jeffrey W. Dalley, Trevor W. Robbins, Barry J. Everitt
Journal of Neuroscience • 2002
Contingent versus Non-contingent Cue Presentation Task
Objective: Investigate dopamine release in the dorsal striatum during cocaine-seeking behavior under the control of a drug-associated cue, comparing contingent versus non-contingent cue presentation
This is a Contingent versus Non-contingent Cue Presentation Task protocol using rat as the model organism. The procedure involves 5 procedural steps, 4 equipment items, 1 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Initial cocaine self-administration training • Progressive increase in response requirement • Microdialysis session with contingent cue presentation
Primary readouts
- Dopamine release in the dorsal striatum during drug-seeking behavior
- Comparison of dopamine levels between contingent and non-contingent cue presentation conditions
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Initial cocaine self-administration training
Rats were trained to self-administer cocaine under a continuous reinforcement schedule where a response on one of two identical levers led to a 20 second presentation of a light CS+ and an intravenous cocaine infusion
Note: Continuous reinforcement schedule used initially
View evidence from paper
“Rats were initially trained to self-administer cocaine under a continuous reinforcement schedule where a response on one of two identical levers led to a 20 sec presentation of a light CS+ and an intravenous cocaine infusion (0.75 mg/kg)”
Progressive increase in response requirement
The response requirement for the CS+ and cocaine was progressively increased until stable responding was established under a second-order schedule of reinforcement
Note: Progression continued until stable responding achieved
View evidence from paper
“The response requirement for the CS+ and cocaine was then progressively increased until stable responding was established under a second-order schedule of reinforcement”
Microdialysis session with contingent cue presentation
During microdialysis, rats were presented with the cocaine-associated CS+ contingent on responding during a session of cocaine-seeking behavior
Note: Contingent presentation means cue appears only when rat responds
View evidence from paper
“During microdialysis, rats were presented with the cocaine-associated CS+ either noncontingently or contingent on responding during a session of cocaine-seeking behavior”
Microdialysis session with non-contingent cue presentation
During microdialysis, rats were presented with the cocaine-associated CS+ non-contingently during a session of cocaine-seeking behavior
Note: Non-contingent presentation means cue appears regardless of responding
View evidence from paper
“During microdialysis, rats were presented with the cocaine-associated CS+ either noncontingently or contingent on responding during a session of cocaine-seeking behavior”
Measure dopamine release
Dopamine release in the dorsal striatum was measured during both contingent and non-contingent cue presentation conditions
Note: Microdialysis was used to measure dopamine levels
View evidence from paper
“in vivo microdialysis in combination with a second-order schedule of cocaine reinforcement was used to investigate the role of the dorsal striatal dopamine innervation”