Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions

Mehrbod Estaki, Jason Pither, Peter Baumeister, Jonathan P. Little, Sandeep K. Gill et al.

Microbiome • 2016

DOI PMC

Dietary Habits Interview

behavioralhuman

Objective: Assess and document participants' dietary habits through verbal interview

Materials & Equipment Checklist

2 items

Gather these items before starting the experiment. Check off items as you prepare.

Equipment1

VO2 peak cycle test equipment

not specified • not specified • not specified • not specified

Amazon

Materials1

Stool collection kit

not specified • not specified • not specified • not specified

Amazon

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Protocol Steps

Participant recruitment and screening

Recruit healthy young adults between 18 and 35 years old. Apply exclusion criteria: no antibiotic treatment within previous 6 months, no current prescribed pharmaceutical drug utilization, and no active acute or chronic diseases

not specifiednot specified

Note: Exclusion criteria must be verified before enrollment

View evidence from paper

“Healthy young adults between 18 and 35 years old were recruited. Exclusion criteria included antibiotic treatment within the previous 6 months, current prescribed pharmaceutical drug utilization, or active acute or chronic diseases”

Verbal dietary habits interview

Conduct a verbal interview with participants to assess and document their dietary habits

not specifiednot specified

Note: Interview method and specific questions not detailed in text

View evidence from paper

“All participants were verbally interviewed on their dietary habits”

Cardiorespiratory fitness assessment

Determine cardiorespiratory fitness (CRF) using a VO2 peak cycle test

not specifiednot specified

Note: Conducted during lab visit

View evidence from paper

“CRF was determined using a VO 2 peak cycle test”

Stool sample collection kit distribution

Provide participants with a stool collection kit and written instructions for proper sample collection

not specifiednot specified

Note: Distributed following lab visit

View evidence from paper

“Participants were then provided a stool collection kit with instructions”

Stool sample collection by participant

Participants collect and return stool sample following the provided instructions

Within one week following lab visitnot specified

Note: Sample must be provided within specified timeframe

View evidence from paper

“were asked to provide a sample within a week following their lab visit”