Source Paper
The amygdala central nucleus and appetitive Pavlovian conditioning: lesions impair one class of conditioned behavior
M Gallagher, PW Graham, PC Holland
Journal of Neuroscience • 1990
Differential Conditioning
Objective: To assess acquisition of CS-generated and US-generated conditioned responses in rats during differential conditioning, and to evaluate the role of the amygdala central nucleus in appetitive Pavlovian conditioning
This is a Differential Conditioning protocol using rat as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 1 materials. Extracted from a 1990 paper published in Journal of Neuroscience.
Model and subjects
rat • not specified • unknown • not specified • not specified
Study window
Estimated timing pending
Core workflow
Surgical lesioning (for lesion group) • Differential conditioning training • Monitoring of conditioned responses
Primary readouts
- Acquisition of CS-generated conditioned responses to visual cues
- Acquisition of CS-generated conditioned responses to auditory cues
- Acquisition of US-generated conditioned responses
- Orienting responses to conditioned stimuli
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Surgical lesioning (for lesion group)
Rats in the lesion condition received neurotoxic lesions of the amygdala central nucleus prior to conditioning
Note: Only lesioned rats underwent this procedure; control rats did not receive lesions
View evidence from paper
“Rats with neurotoxic lesions of the amygdala central nucleus (CN) were trained using appetitive Pavlovian conditioning procedures”
Differential conditioning training
Rats received reinforced presentations of one sensory cue (either visual or auditory) paired with food, and nonreinforced presentations of the other cue. Half of each lesion condition received reinforced visual/nonreinforced auditory; the other half received reinforced auditory/nonreinforced visual
Note: Both lesioned and unlesioned rats underwent this training with counterbalanced cue assignments
View evidence from paper
“During differential conditioning, some lesioned and unlesioned rats received reinforced presentations of a visual cue and nonreinforced presentations of an auditory cue, and the others in each lesion condition received reinforced auditory and nonreinforced visual cue presentations”
Monitoring of conditioned responses
Two classes of conditioned responses were monitored: CS-generated CRs (resembling orienting responses elicited by CSs prior to pairing) and US-generated CRs (resembling behavior elicited by food reinforcement itself)
Note: Responses were recorded during conditioning trials to assess acquisition
View evidence from paper
“Conditioned responses (CRs) that are representative of 2 classes of behavior were monitored. One type of CR resembled the orienting responses that were elicited by the conditioned stimuli (CSs) prior to pairing with food reinforcement: the other type of CR resembled the behavior elicited by food reinforcement itself”
Assessment of orienting responses and habituation
Orienting responses and habituation to the conditioned stimuli were evaluated and compared between lesion and control groups
Note: These measures were used to determine if differences in CR acquisition were due to general sensory or motor deficits
View evidence from paper
“Orienting responses and habituation to the CSs were, however, comparable for the lesion and control groups”