Source Paper
Reduced fear expression after lesions of the ventral hippocampus
Kirsten G. Kjelstrup, Frode A. Tuvnes, Hill-Aina Steffenach, Robert Murison, Edvard I. Moser et al.
Proceedings of the National Academy of Sciences • 2002
Elevated Plus-Maze
Objective: Assessment of innate fear and anxiety-related behavior by measuring avoidance of open arms in an elevated plus-maze
This is a Elevated Plus-Maze protocol using rat as the model organism. The procedure involves 2 procedural steps, 2 equipment items. Extracted from a 2002 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Elevated plus-maze test • Brightly lit chamber confinement test
Primary readouts
- Avoidance of open arms in elevated plus-maze
- Neuroendocrine stress responses during confinement to brightly lit chamber
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Elevated plus-maze test
Rats are placed in an elevated plus-maze to assess avoidance of open arms as a measure of innate fear and anxiety-related behavior
Note: Measurement of open arm avoidance indicates fear-related behavior
View evidence from paper
“Rats with selective hippocampal lesions failed to avoid open arms in an elevated plus-maze”
Brightly lit chamber confinement test
Rats are confined to a brightly lit chamber and neuroendocrine stress responses are measured
Note: Lesioned rats showed decreased neuroendocrine stress responses
View evidence from paper
“had decreased neuroendocrine stress responses during confinement to a brightly lit chamber”