Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Elevated Plus Maze
Objective: To assess anxiety-related behavior in mice
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
Estimated: $1,890.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Set up apparatus
Use apparatus with four arms (closed and open, 30 × 6 cm each) with central area (6 × 6 cm). Closed arms have 20-cm-high walls. Elevate device 60 cm above floor.
View evidence from paper
“four arms connected perpendicularly (closed and open, 30 × 6 cm each) with a central area (6 × 6 cm)”
Set up lighting
Provide dim illumination with diffuse lighting at 100 lx using 25 W halogen lamp placed under the device.
View evidence from paper
“dimly illuminated with diffuse lighting (100 lx) of a halogen lamp (25 W) placed under the device”
Connect tracking system
Connect 3D sensor Kinect 1 to PC through USB-2 port for automatic movement tracking.
View evidence from paper
“animal's movements were automatically traced with a 3D sensor Kinect 1 connected to the PC through a USB-2 port”
Conduct test
Test animal for 5 minutes while sensor automatically detects movement in open and closed arms.
View evidence from paper
“During the 5 min of testing, the sensor automatically detected the animal's movement in the open as well as the closed arms”
Record manual observations
Count stretch poses and head dips from the open arms using experienced rater.
View evidence from paper
“Stretch poses and head dips from the open arms were counted by an experienced rater”
Clean apparatus
Clean arena with wet H2O2 and dry napkins after each test.
View evidence from paper
“The arena was cleaned with wet (H2O2) and dry napkins after each test”