Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Elevated Plus Maze
Objective: To assess anxiety-related behavior in mice
This is a Elevated Plus Maze protocol using Mice as the model organism. The procedure involves 6 procedural steps, 4 equipment items, 2 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
Mice • C57BL/6-Ptpn5_KO and C57BL/6 • Male • 2-month-old • Not specified
Study window
~5 minutes hands-on
Core workflow
Set up apparatus • Set up lighting • Connect tracking system
Primary readouts
- Total path (m)
- Time (%) spent in the center
- Time (%) spent in open arms
- Time (%) spent in closed arms
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Set up apparatus
Use apparatus with four arms (closed and open, 30 × 6 cm each) with central area (6 × 6 cm). Closed arms have 20-cm-high walls. Elevate device 60 cm above floor.
View evidence from paper
“four arms connected perpendicularly (closed and open, 30 × 6 cm each) with a central area (6 × 6 cm)”
Set up lighting
Provide dim illumination with diffuse lighting at 100 lx using 25 W halogen lamp placed under the device.
View evidence from paper
“dimly illuminated with diffuse lighting (100 lx) of a halogen lamp (25 W) placed under the device”
Connect tracking system
Connect 3D sensor Kinect 1 to PC through USB-2 port for automatic movement tracking.
View evidence from paper
“animal's movements were automatically traced with a 3D sensor Kinect 1 connected to the PC through a USB-2 port”
Conduct test
Test animal for 5 minutes while sensor automatically detects movement in open and closed arms.
View evidence from paper
“During the 5 min of testing, the sensor automatically detected the animal's movement in the open as well as the closed arms”
Record manual observations
Count stretch poses and head dips from the open arms using experienced rater.
View evidence from paper
“Stretch poses and head dips from the open arms were counted by an experienced rater”
Clean apparatus
Clean arena with wet H2O2 and dry napkins after each test.
View evidence from paper
“The arena was cleaned with wet (H2O2) and dry napkins after each test”