Source Paper
Stress duration modulates the spatiotemporal patterns of spine formation in the basolateral amygdala
Rupshi Mitra, Shantanu Jadhav, Bruce S. McEwen, Ajai Vyas, Sumantra Chattarji
Proceedings of the National Academy of Sciences • 2005
Elevated Plus-Maze
Objective: Assessment of anxiety-like behavior in rats following acute immobilization stress using elevated plus-maze testing
This is a Elevated Plus-Maze protocol using rat as the model organism. The procedure involves 2 procedural steps, 1 equipment items. Extracted from a 2005 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
rat • not specified • male • not specified • not specified
Study window
~1.4 week study window
Core workflow
Acute Immobilization Stress (AIS) Administration • Elevated Plus-Maze Testing at 10 Days Post-Stress
Primary readouts
- Anxiety-like behavior on elevated plus-maze
- Open vs closed arm exploration patterns
- Temporal development of anxiety symptoms following acute stress
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Acute Immobilization Stress (AIS) Administration
Rats exposed to acute immobilization stress as aversive experience
Note: Single exposure to immobilization stress
View evidence from paper
“aversive experience in the form of acute immobilization stress (AIS)”
Elevated Plus-Maze Testing at 10 Days Post-Stress
Behavioral assessment of anxiety-like behavior on elevated plus-maze conducted 10 days after acute immobilization stress exposure
Note: Testing performed 10 days after AIS to measure delayed anxiety development
View evidence from paper
“gradual development of anxiety-like behavior on the elevated plus-maze 10 d after AIS”